Analytical Data
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Gene name
CASP1
- Application
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Alternative Names
ICE; IL1BC; IL1-BC; P45; Interleukin 1 Beta Converting Enzyme; Apoptosis-Related Cysteine Peptidase; Interleukin-1 beta convertase
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Species
Rat
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Source
E. coli
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Tag
N-His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P43527
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Expression Region
Asn119~Asp296
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Molecular Weight
21kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
Caspase-1 (CASP1) is a crucial enzyme in the inflammatory response, primarily involved in the maturation of pro-inflammatory cytokines such as interleukin-1β (IL-1β) and interleukin-18 (IL-18). Research into recombinant CASP1 proteins has gained significant interest due to its pivotal role in various pathological conditions, including autoimmune diseases, neurodegenerative disorders, and infectious diseases. The activation of CASP1 is associated with the formation of inflammasomes, multiprotein complexes that sense pathogenic microorganisms and stress signals, leading to pyroptosis—a form of programmed cell death. Given its essential functions, CASP1 is a promising target for therapeutic intervention, particularly in inflammatory diseases where its dysregulation contributes to tissue damage and chronic inflammation. The production of recombinant CASP1 proteins facilitates in-depth studies of its enzymatic activity, regulatory mechanisms, and interactions with other cellular components. This research not only enhances our understanding of CASP1's biological functions but also aids in the development of novel therapeutic strategies aimed at modulating its activity to alleviate inflammation-related diseases. Advances in biotechnological methods for generating purified CASP1 proteins have further enabled researchers to explore its structure-function relationship, paving the way for innovative approaches in treating conditions exacerbated by excessive inflammatory responses. Through these endeavors, the study of CASP1 serves as a foundation for illuminating the complexities of inflammation and its regulation in human health and disease.
