Analytical Data
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Gene name
CK12
- Application
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Alternative Names
KRT12; K12; Keratin 12; Meesmann Corneal Dystrophy; Keratin, type I cytoskeletal 12
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Species
Rat
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Source
E. coli
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Tag
N-His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q6IFW5
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Expression Region
Asp398~Met456
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Molecular Weight
19kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
CK12, a member of the keratin family, is a type of intermediate filament protein predominantly found in epithelial tissues. Research into CK12 has gained significant attention due to its potential role in various eye diseases, particularly in maintaining corneal integrity. Mutations in the CK12 gene have been linked to specific corneal dystrophies and other ocular conditions, highlighting its importance in cellular architecture and stability. The study of CK12 recombinant proteins enables scientists to investigate its structural properties and functional mechanisms in a controlled environment. Recombinant CK12 proteins can be produced in various expression systems, allowing for the assessment of their biochemical characteristics, interactions with other cellular components, and contributions to tissue integrity. Furthermore, understanding the pathogenic mechanisms associated with CK12 mutations can provide insights into novel therapeutic strategies for corneal and other epithelial disorders. Thus, the development and characterization of CK12 recombinant proteins are essential for advancing our knowledge of corneal biology and potential clinical applications in treating related diseases.
