Analytical Data
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基因名
CK12
- Application
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别名
KRT12; K12; Keratin 12; Meesmann Corneal Dystrophy; Keratin, type I cytoskeletal 12
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种属
Rat
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表达系统
E. coli
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标签
N-His
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q6IFW5
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表达区间
Asp398~Met456
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分子量
19kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
CK12, a member of the keratin family, is a type of intermediate filament protein predominantly found in epithelial tissues. Research into CK12 has gained significant attention due to its potential role in various eye diseases, particularly in maintaining corneal integrity. Mutations in the CK12 gene have been linked to specific corneal dystrophies and other ocular conditions, highlighting its importance in cellular architecture and stability. The study of CK12 recombinant proteins enables scientists to investigate its structural properties and functional mechanisms in a controlled environment. Recombinant CK12 proteins can be produced in various expression systems, allowing for the assessment of their biochemical characteristics, interactions with other cellular components, and contributions to tissue integrity. Furthermore, understanding the pathogenic mechanisms associated with CK12 mutations can provide insights into novel therapeutic strategies for corneal and other epithelial disorders. Thus, the development and characterization of CK12 recombinant proteins are essential for advancing our knowledge of corneal biology and potential clinical applications in treating related diseases.
