Analytical Data
-
Gene name
PKCg
- Application
-
Alternative Names
PKC-G; PRKCG; PKCC; SCA14; PKC-Gamma
-
Species
Rat
-
Source
E. coli
-
Tag
N-His
-
Purity
Greater than 90% as determined by SDS-PAGE.
-
Uniprot
P63319
-
Expression Region
Phe351~Phe614
-
Molecular Weight
36kDa
-
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
-
Form
Freeze-dried powder
-
Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
-
Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
The PKCγ (Protein Kinase C gamma) protein has garnered significant interest in biomedical research due to its pivotal role in various cellular processes, including signal transduction, neural development, and synaptic plasticity. It is primarily expressed in the brain and is involved in neurotransmitter release, neuronal growth, and apoptosis. Dysregulation of PKCγ has been implicated in several neurodegenerative disorders such as Alzheimer's disease, multiple sclerosis, and epilepsy, highlighting its potential as a therapeutic target. The study of recombinant PKCγ proteins has proven essential in elucidating the molecular mechanisms underlying its function and interactions with other cellular molecules. By producing and analyzing recombinant forms of PKCγ, researchers aim to dissect its enzymatic activity, spatial and temporal distribution, and modulation by various cofactors. Additionally, this research aids in the development of specific inhibitors or activators, which could serve as useful tools in therapeutic strategies for related diseases. Through structural and functional analyses, including crystallography and binding assays, scientists strive to create a comprehensive understanding of PKCγ’s role within the broader context of cell signaling and pathology. Overall, the investigation of PKCγ recombinant proteins is crucial for advancing our knowledge of neuronal function and paving the way for potential clinical applications in treating debilitating neurological conditions.
