Analytical Data
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Gene name
AQP4
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简介
The AQP4 protein forms water-specific channels that are critical for brain water homeostasis and lymphoid solute transport. It regulates water exchange at the blood-brain interface, promoting the influx of cerebrospinal fluid into the brain and the drainage of interstitial fluid. AQP4 Protein, Mouse (Cell-Free) is the recombinant mouse-derived AQP4 protein, expressed by E. coli Cell-free , with tag free.
- Application
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Alternative Names
Aquaporin-4; Mercurial-insensitive water channel; MIWC; WCH4
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Species
Mouse
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Source
E. coli Cell-free
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Tag
Tag Free
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P55088
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Expression Region
M1-V323
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AA Sequence
MSDGAAARRWGKCGHSCSRESIMVAFKGVWTQAFWKAVSAEFLATLIFVLLGVGSTINWGGSENPLPVDMVLISLCFGLSIATMVQCFGHISGGHINPAVTVAMVCTRKISIAKSVFYIIAQCLGAIIGAGILYLVTPPSVVGGLGVTTVHGNLTAGHGLLVELIITFQLVFTIFASCDSKRTDVTGSIALAIGFSVAIGHLFAINYTGASMNPARSFGPAVIMGNWANHWIYWVGPIMGAVLAGALYEYVFCPDVELKRRLKEAFSKAAQQTKGSYMEVEDNRSQVETEDLILKPGVVHVIDIDRGEEKKGKDSSGEVLSSV
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Protein Length
Full Length
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Molecular Weight
Observed band size: Monomer: 34 kDa Dimer: 68 kDa It is speculated that the protein forms a dimeric structure.
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Aquaporin-4 (AQP4) is a critical water channel protein predominantly expressed in the brain, particularly in astrocytes, where it facilitates water transport across cell membranes. The importance of AQP4 in maintaining brain homeostasis and regulating water balance has drawn significant attention in recent years, with implications for various neurological conditions, including brain edema, multiple sclerosis, and neurodegenerative diseases. Research on recombinant AQP4 proteins aims to elucidate their structure, function, and regulatory mechanisms, which are essential for understanding their role in physiological and pathological processes. Recombinant production of AQP4 allows for detailed biochemical studies, structural analysis through techniques such as X-ray crystallography and cryo-electron microscopy, and the development of specific inhibitors or modulators that can influence AQP4 activity. These investigations not only enhance our comprehension of water transport dynamics in the central nervous system but also pave the way for potential therapeutic strategies targeting AQP4-related disorders. As the field continues to advance, the development of hybrid proteins and novel delivery systems for AQP4 could further enhance our understanding and manipulation of this vital channel, offering promising avenues for research and clinical application.
