Analytical Data
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Gene name
CRKL
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简介
CRKL protein mediates intracellular signal transduction, interacts with tyrosine-phosphorylated EPOR and INPP5D/SHIP1, and plays a role in erythropoietin receptor and phosphoinositide signaling. It also binds to DOCK2 and DOCK5, suggesting regulation of guanine nucleotide exchange factors. CRKL Protein, Human is the recombinant human-derived CRKL protein, expressed by E. coli , with tag free.
- Application
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Alternative Names
CRKL; Crk-like protein
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Species
Human
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Source
E. coli
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Tag
Tag Free
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P46109
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Expression Region
M1-E303
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Protein Length
Full Length
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Molecular Weight
33.9 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
CRKL, or CRK-like protein, is a member of the CRK gene family known for its role in mediating signal transduction pathways that influence cellular processes such as proliferation, differentiation, and survival. It plays a critical role in various biological functions, including cytokine signaling and cell migration, making it a key player in both normal physiology and disease states, particularly in cancer. Recent research has highlighted CRKL's involvement in oncogenic signaling pathways, as it acts as an adapter protein interacting with several tyrosine kinases and other signaling molecules. The study of CRKL recombinant proteins is essential for elucidating its biological functions and regulatory mechanisms. By expressing and purifying CRKL in a recombinant system, researchers can investigate its interactions with other proteins, identify potential post-translational modifications, and analyze its functional roles in vitro and in vivo. Understanding the structure-function relationship of CRKL through recombinant studies can provide insights into its contribution to tumorigenesis and may lead to the identification of novel therapeutic targets for cancer treatment. As such, CRKL represents a promising avenue for research aimed at unraveling the complexities of cell signaling networks and their implications in health and disease.
