Analytical Data
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Gene name
USAG-1
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简介
USAG-1 protein is involved in initiating endometrial receptivity, contributing to implantation preparation and decidual cell response sensitization. It regulates signaling pathways by enhancing Wnt signaling while inhibiting TGF-β signaling. USAG-1 Protein, Human (HEK293, Fc) is the recombinant human-derived USAG-1 protein, expressed by HEK293 , with C-hFc labeled tag.
- Application
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Biological Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human USAG1 is coated at 2 μg/mL (100 μL/well), the concentration of rhLRP-6 that produces 50% of the optimal binding response is found to be approximately 0.0323 μg/mL. Measured by its binding ability in a functional ELISA. When Recombinant Human USAG1 is coated at 2 μg/mL (100 μL/well), the concentration of rhLRP-6 that produces 50% of the optimal binding response is found to be approximately 0.0323 μg/mL.
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Alternative Names
Uterine sensitization-associated gene 1 protein
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Species
Human
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Source
HEK293
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Tag
C-hFc
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Purity
Greater than 95% as determined by SDS-PAGE.
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Uniprot
Q6X4U4-1
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Expression Region
F24-S206
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AA Sequence
FKNDATEILYSHVVKPVPAHPSSNSTLNQARNGGRHFSNTGLDRNTRVQVGCRELRSTKYISDGQCTSISPLKELVCAGECLPLPVLPNWIGGGYGTKYWSRRSSQEWRCVNDKTRTQRIQLQCQDGSTRTYKITVVTACKCKRYTRQHNESSHNFESMSPAKPVQHHRERKRASKSSKHSMS
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Protein Length
Full Length of Isoform-1 Mature Protein
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Molecular Weight
55-70 kDa.
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
USAG-1 (Ubiquitously Expressed Secreted Protein 1) is a type of secreted protein that plays a critical role in various biological processes, particularly in the regulation of tissue growth and development. In recent years, significant interest has developed around USAG-1 due to its involvement in signaling pathways associated with bone and tooth formation, making it a potential target for therapeutic strategies in regenerative medicine and dental applications. Research has indicated that USAG-1 acts as an antagonist of the bone morphogenetic protein (BMP) signaling pathway, which is pivotal in the regulation of osteogenesis and chondrogenesis. Elevated levels of USAG-1 have been associated with certain pathological conditions, such as osteoporosis and other degenerative bone diseases, prompting investigations into its mechanism of action and potential as a biomarker. Additionally, understanding the structural and functional properties of USAG-1 could provide insights into its interactions with other signaling molecules. The recombinant expression and purification of USAG-1 have proven essential for functional studies, making it a valuable subject for further experimental investigations to elucidate its role in health and disease, as well as to explore its potential applications in therapeutic development. Overall, the study of USAG-1 not only enhances our knowledge of skeletal biology but also opens new avenues for innovative treatments in bone-related disorders.











