Analytical Data
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Gene name
MCAK
- Application
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Alternative Names
KIF2C; KNSL6; Kinesin Family Member 2C; Kinesin-Like 6
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Species
Human
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Source
HEK293
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Tag
N-His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q99661
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Expression Region
Ala2~Gln725
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Molecular Weight
83kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
MCAK, or Mitotic Centromere-Associated Kinesin, is a member of the kinesin superfamily of motor proteins, primarily known for its role in the regulation of microtubule dynamics during cell division. Research on MCAK has gained significant attention due to its essential function in mitosis and its implication in various diseases, including cancer. The protein acts as a depolymerase that regulates the stability of microtubules at the centromere, facilitating the proper segregation of chromosomes during cell division. Dysregulation of MCAK can lead to chromosome misalignment and aneuploidy, which are common features in tumorigenesis. Understanding the structural and functional properties of MCAK reconstituted as a recombinant protein presents opportunities for elucidating its molecular mechanisms and interactions with other cellular components. Such studies are crucial for developing targeted therapies aimed at modulating MCAK activity, thereby enhancing the efficacy of cancer treatment. The advancements in recombinant protein technology enable detailed investigations into MCAK's role in cell cycle regulation, making it a pivotal subject in cancer research and therapeutic development.
