Analytical Data
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Gene name
Coagulation Factor X/F10
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简介
Factor X (F10) is a vitamin K-dependent glycoprotein that plays a key role in the blood coagulation process as factor Xa. Factor Xa, along with factor Va, calcium, and phospholipids, coordinates the conversion of prothrombin to thrombin, a key step in the coagulation cascade. Coagulation Factor X/F10 Protein, Human (HEK293, Fc) is the recombinant human-derived Coagulation Factor X/F10 protein, expressed by HEK293 , with C-hFc labeled tag.
- Application
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Alternative Names
rHuCoagulation Factor X/F10, Fc; Coagulation factor X; Stuart factor; Stuart-Prower factor
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Species
Human
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Source
HEK293
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Tag
C-hFc
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P00742
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Expression Region
N32-K488
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AA Sequence
NNILARVTRANSFLEEMKKGHLERECMEETCSYEEAREVFEDSDKTNEFWNKYKDGDQCETSPCQNQGKCKDGLGEYTCTCLEGFEGKNCELFTRKLCSLDNGDCDQFCHEEQNSVVCSCARGYTLADNGKACIPTGPYPCGKQTLERRKRSVAQATSSSGEAPDSITWKPYDAADLDPTENPFDLLDFNQTQPERGDNNLTRIVGGQECKDGECPWQALLINEENEGFCGGTILSEFYILTAAHCLYQAKRFKVRVGDRNTEQEEGGEAVHEVEVVIKHNRFTKETYDFDIAVLRLKTPITFRMNVAPACLPERDWAESTLMTQKTGIVSGFGRTHEKGRQSTRLKMLEVPYVDRNSCKLSSSFIITQNMFCAGYDTKQEDACQGDSGGPHVTRFKDTYFVTGIVSWGEGCARKGKYGIYTKVTAFLKWIDRSMKTRGLPKAKSHAPEVITSSPLK
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Protein Length
Full Length of Mature Protein
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Molecular Weight
80-130 & 22-25 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
Coagulation Factor X, also known as F10, is a crucial component of the coagulation cascade, playing a vital role in hemostasis by converting prothrombin to thrombin, which subsequently leads to fibrin formation and blood clotting. Deficiencies in Factor X can result in bleeding disorders, highlighting the need for therapeutic interventions. Recombinant Factor X proteins have emerged as significant biopharmaceuticals, offering advantages over plasma-derived proteins, such as greater safety, reduced risk of viral transmission, and enhanced stability. The development of recombinant Factor X has been driven by advancements in biotechnology, including genetic engineering techniques and the use of mammalian cell systems for protein production. Research into recombinant F10 has focused on optimizing its functional properties, improving its pharmacokinetics, and enhancing its efficacy in treating coagulopathies. Moreover, novel formulations and delivery methods are being explored to maximize patient outcomes, particularly in the context of hemophilia and congenital Factor X deficiency. As the understanding of Factor X's role in the coagulation pathway deepens, ongoing studies aim to fine-tune its therapeutic applications, paving the way for more effective and personalized treatment strategies for patients affected by bleeding disorders.
