Analytical Data
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Gene name
Blot21
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简介
Blot21 Protein, representing a new group of allergens, is an important Blomia tropicalis allergen. Allergenic components from Blomia tropicalis are important triggers of allergies in the tropics. Blot21 is a paralog of the group 5 allergen Blot5. Blot21 has moderate sequence identity (40.7%) to Blot5 and low to moderate cross-reactivity to Blot5. Blot21 Protein, Blomia tropicalis (His) is the recombinant Blot21 protein, expressed by E. coli , with N-6*His labeled tag.
- Application
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Alternative Names
Mite allergen Blo t 21; Mite group 21 allergen Blo t 21; Blo t 21
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Species
Others
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Source
E. coli
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Tag
N-6*His
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Purity
Greater than 95% as determined by SDS-PAGE.
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Uniprot
AAX34047.1
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Expression Region
L17-E129
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Protein Length
Partial
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Molecular Weight
13.5 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Blot21 is a recombinant protein that has garnered significant attention in the field of molecular biology and immunology. Research into Blot21 arises from its potential application in various therapeutic and diagnostic settings. The protein, derived from specific pathogens, is believed to play a crucial role in eliciting immune responses, making it a valuable candidate for vaccine development and immunotherapy. Studies have demonstrated that Blot21 can enhance the activation of immune cells, thereby improving recognition and targeting of infected or malignant cells. Additionally, its unique structural properties allow for the development of novel diagnostic assays, aiding in the detection of related diseases. The exploration of Blot21's function and potential applications is critical, not only for understanding its biological mechanisms but also for leveraging its capabilities in combating infectious diseases and cancer. As researchers continue to examine the interactions of Blot21 with the immune system, the insights gained may lead to breakthroughs in enhancing vaccine efficacy and developing innovative therapeutic strategies. With the increasing demand for effective medical interventions, Blot21 represents a promising avenue for advancing both research and clinical applications in immunology.
