Analytical Data
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基因名
Enolase 1/ENO1
- Application
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别名
NNE; ENO1L1; MPB1; PPH; Alpha Enolase; Enolase 1; Phosphopyruvate hydratase; Plasminogen-binding protein; 2-phospho-D-glycerate hydro-lyase; C-myc promoter-binding
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种属
Mouse
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表达系统
E. coli
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标签
N-His
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P17182
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表达区间
Met1~Lys434
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分子量
48kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Enolase 1 (ENO1), also known as alpha-enolase, is a glycolytic enzyme that plays a crucial role in carbohydrate metabolism by catalyzing the conversion of 2-phosphoglycerate to phosphoenolpyruvate, a key step in the glycolytic pathway. Beyond its metabolic functions, ENO1 is implicated in various cellular processes, including cell proliferation, differentiation, and response to stress. Research has shown that ENO1 is overexpressed in numerous cancers, suggesting a potential role in tumorigenesis and cancer progression, making it a target of interest for cancer diagnostics and therapeutics. Moreover, ENO1 exhibits moonlighting properties, functioning also as a moonlighting protein in processes such as immune responses and cytoskeletal organization. The production of recombinant ENO1 protein has facilitated the exploration of its structure-function relationships, interactions with other proteins, and mechanistic insights into its dual roles in metabolism and signaling pathways. Understanding the biological implications of ENO1 can not only enhance our knowledge of metabolic regulation but also pave the way for novel therapeutic strategies, particularly in oncology. As researchers continue to investigate ENO1's diverse functions and therapeutic potentials, the production and characterization of ENO1 recombinant protein remain essential for unveiling its roles in health and disease.












