Analytical Data
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Gene name
TPST1
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简介
TPST1 (tyrosyl protein sulfotransferase 1) catalyzes the O-sulfation of tyrosine residues located within the acidic motifs of polypeptides, utilizing 3'-phosphoadenylate sulfate (PAPS) as a co-substrate. This enzymatic activity plays a crucial role in post-translational modification processes, especially sulfation, which contributes to the structural and functional diversity of proteins. TPST1 Protein, Human (HEK293, His) is the recombinant human-derived TPST1, expressed by HEK293 , with N-His labeled tag. ,
- Application
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Alternative Names
Protein-tyrosine sulfotransferase 1; Tyrosylprotein sulfotransferase 1; TPST-1
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Species
Human
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Source
HEK293
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Tag
N-His
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Purity
Greater than 95% as determined by SDS-PAGE.
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Uniprot
O60507
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Expression Region
Q26-E370
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AA Sequence
QHAMECHHRIEERSQPVKLESTRTTVRTGLDLKANKTFAYHKDMPLIFIGGVPRSGTTLMRAMLDAHPDIRCGEETRVIPRILALKQMWSRSSKEKIRLDEAGVTDEVLDSAMQAFLLEIIVKHGEPAPYLCNKDPFALKSLTYLSRLFPNAKFLLMVRDGRASVHSMISRKVTIAGFDLNSYRDCLTKWNRAIETMYNQCMEVGYKKCMLVHYEQLVLHPERWMRTLLKFLQIPWNHSVLHHEEMIGKAGGVSLSKVERSTDQVIKPVNVGALSKWVGKIPPDVLQDMAVIAPMLAKLGYDPYANPPNYGKPDPKIIENTRRVYKGEFQLPDFLKEKPQTEQVE
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Protein Length
Lumenal Domain
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Molecular Weight
43-57 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
TPST1 (Tyrosylprotein Sulfotransferase 1) is an essential enzyme involved in the post-translational modification of proteins through the addition of sulfate groups to tyrosine residues. This modification is critical for various biological processes, including protein folding, stability, and interactions. The study of TPST1 has gained significance due to its role in modulating receptor activity, influencing cellular signaling pathways, and contributing to various physiological functions. Dysregulation of TPST1 has been linked to several diseases, including cancer and neurodegenerative disorders, highlighting its potential as a therapeutic target. Research into recombinant TPST1 focuses on understanding its catalytic mechanisms, substrate specificity, and interactions with other cellular components. By producing TPST1 as a recombinant protein, scientists can explore its functional characteristics and role in tyrosine sulfation more effectively. This knowledge could pave the way for novel therapeutics and diagnostic tools, as well as contribute to the broader understanding of post-translational modifications in health and disease. The ongoing investigation of TPST1 and its recombinant forms is expected to unveil critical insights into its biochemical pathways and enhanced therapeutic strategies.
