Analytical Data
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Gene name
MD-2/LY96
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简介
MD-2/LY96 Protein, acting as a monomer, robustly and specifically binds to interleukin-13 (IL13), distinguishing it from interleukin-4 (IL4) with which it does not interact.This selective binding highlights MD-2/LY96's pivotal role in mediating cellular responses to IL13, underscoring its significance in IL13-related biological activities.MD-2/LY96 Protein, Mouse (HEK293, Fc) is the recombinant mouse-derived MD-2/LY96 protein, expressed by HEK293 , with C-hFc labeled tag.
- Application
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Biological Activity
Measured by its binding ability in a functional ELISA. When recombinant human TLR-4 is Immobilized at 2 µg/mL (100 µL/well), can bind Biotinylated MD-2. The ED50 for this effect is 1.491 μg/mL. Measured by its binding ability in a functional ELISA. When recombinant human TLR-4 is Immobilized at 2 µg/mL (100 µL/well),can bind Biotinylated MD-2. The ED50 for this effect is 1.491 μg/mL.
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Alternative Names
Lymphocyte antigen 96; Ly-96; ESOP-1
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Species
Mouse
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Source
HEK293
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Tag
C-hFc
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Purity
Greater than 95% as determined by SDS-PAGE.
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Uniprot
Q9JHF9
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Expression Region
E19-N160
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Protein Length
Full Length of Mature Protein
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Molecular Weight
47 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
MD-2, also known as LY96, is a key accessory protein that plays a crucial role in the innate immune response by forming a complex with Toll-like receptor 4 (TLR4). This complex is essential for the recognition of lipopolysaccharides (LPS), which are components of the outer membrane of Gram-negative bacteria. When LPS binds to the MD-2/TLR4 complex, it triggers a cascade of signaling pathways that lead to the activation of immune cells, culminating in the production of pro-inflammatory cytokines. Understanding MD-2/LY96 is vital for elucidating the mechanisms of sepsis and other inflammatory diseases, as it could pave the way for novel therapeutic interventions. Research has shown that variations in the MD-2 gene can affect susceptibility to infections and the inflammatory response, making it an important target for study. Additionally, recombinant MD-2 protein has been utilized in various experimental settings to explore its structure-function relationships and to develop potential inhibitors that could modulate TLR4 signaling. The ongoing investigation into MD-2/LY96 not only enhances our understanding of immune system dynamics but also contributes to the development of strategies for treating bacterial infections and related inflammatory conditions.
