Analytical Data
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Gene name
MCEMP1
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简介
MCEMP1, a lung-specific surface protein, is a type II transmembran protein. MCEMP1 is primarily expressed in myeloid lineage immune cells and plays a critical in allergic and inflammatory lung diseases. MCEMP1 is an adaptor for KIT receptor to promotes stem cell factor-mediated mast cell proliferation. MCEMP1 also participates in the chemotaxis, adhesion, and migration of circulating monocytes[1][2]. MCEMP1 Protein, Human (HEK293, Fc) is the recombinant human-derived MCEMP1 protein, expressed by HEK293 , with N-mFc labeled tag.
- Application
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Biological Activity
Measured by the ability of the immobilized protein to support the adhesion of THP-1 human acute monocytic leukemia cells. The ED50 for this effect is 0.2521 μg/mL, corresponding to a specific activity is 3.967×10^3 units/mg. Measured by the ability of the immobilized protein to support the adhesion of THP-1 human acute monocytic leukemia cells. The ED50 for this effect is 0.2521 μg/mL, corresponding to a specific activity is 3.967×103 units/mg.
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Alternative Names
Mast cell-expressed membrane protein 1; C19orf59
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Species
Human
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Source
HEK293
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Tag
N-mFc
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q8IX19-1
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Expression Region
K107-Q187
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Protein Length
Extracellular Domain
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Molecular Weight
40-50 kDa.
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
MCEMP1 (Mast Cell-Enhanced Membrane Protein 1) is a protein that has garnered interest in immunology and cell biology due to its potential role in mast cell function and immune responses. Initially identified as a protein enriched in mast cells, MCEMP1 is thought to be involved in allergic reactions and inflammation. Research has suggested that it may influence mast cell survival, activation, and the secretion of pro-inflammatory mediators. Studies have shown that MCEMP1 expression is modulated by various cytokines, indicating its potential role in immune signaling pathways. Furthermore, the characterization of MCEMP1 as a recombinant protein allows for the exploration of its functional properties and interactions with other cellular components. Understanding the biological mechanisms behind MCEMP1 can provide insights into its implications in allergic diseases and could potentially lead to novel therapeutic strategies for managing mast cell-related conditions. Ongoing research aims to elucidate its structure-function relationship and its relevance in both normal and pathological immune responses, emphasizing the need for continued investigation into this intriguing protein.
