Analytical Data
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基因名
Carbonic Anhydrase 1
- Application
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别名
rHuCarbonic Anhydrase 1, His; Carbonic Anhydrase 1; Carbonate Dehydratase I; Carbonic Anhydrase B; CAB; Carbonic Anhydrase I; CA1
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种属
Human
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表达系统
E. coli
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标签
C-6*His
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纯度
Greater than 95% as determined by SDS-PAGE.
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蛋白编号
P00915
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表达区间
A2-F261
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氨基酸序列
ASPDWGYDDKNGPEQWSKLYPIANGNNQSPVDIKTSETKHDTSLKPISVSYNPATAKEIINVGHSFHVNFEDNDNRSVLKGGPFSDSYRLFQFHFHWGSTNEHGSEHTVDGVKYSAELHVAHWNSAKYSSLAEAASKADGLAVIGVLMKVGEANPKLQKVLDALQAIKTKGKRAPFTNFDPSTLLPSSLDFWTYPGSLTHPPLYESVTWIICKESISVSSEQLAQFRSLLSNVEGDNAVPMQHNNRPTQPLKGRTVRASFHHHHHH
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蛋白长度
Full Length of Mature Protein
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分子量
25-35 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Carbonic Anhydrase 1 (CA1) is an enzyme that has garnered significant attention in biochemical and medical research due to its pivotal role in the regulation of pH and CO2 concentration in biological systems. This enzyme catalyzes the reversible hydration of carbon dioxide to bicarbonate and protons, which is crucial for various physiological processes, including respiration, acid-base balance, and photosynthesis in plants. Its dysfunction has been linked to numerous pathological conditions, including glaucoma, epilepsy, and certain types of cancer, making it a potential target for therapeutic intervention. The recombinant expression of CA1 provides a valuable tool for studying its structure-function relationships and developing inhibitors that could serve as drugs. Advances in molecular cloning and protein expression systems have enabled researchers to produce large quantities of highly purified CA1 for kinetic studies, structural analysis, and drug screening. Understanding the enzymatic mechanisms and regulation of CA1 at the molecular level can contribute to the development of novel pharmacological agents and enhance our comprehension of its biological significance in health and disease. Additionally, the study of CA1 is not only vital for its implications in human health but also offers insights into the evolutionary aspects of carbonic anhydrases across different organisms, illuminating their diverse functions and adaptations. Overall, research on recombinant CA1 is essential for both basic science and the potential development of clinical applications aimed at modulating its activity for therapeutic benefits.
