Analytical Data
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基因名
ACSS2
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简介
The ACSS2 protein plays a key role in catalyzing the synthesis of acetyl-CoA from short-chain fatty acids, with acetate being the preferred substrate. Although acetate is efficiently utilized, ACSS2 can also process propionate, albeit with significantly lower affinity. ACSS2 Protein, Human (His) is the recombinant human-derived ACSS2 protein, expressed by E. coli , with N-6*His labeled tag.
- Application
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种属
Human
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表达系统
E. coli
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标签
N-6*His
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q9NR19-1
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表达区间
G2-Q701
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蛋白长度
Partial
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分子量
80.5 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
ACSS2 (Acetyl-CoA synthetase 2) is an essential enzyme involved in cellular metabolism, particularly in the conversion of acetate into acetyl-CoA, a critical substrate for various metabolic pathways, including fatty acid synthesis and the TCA cycle. Research into ACSS2 has gained attention due to its role in regulating energy homeostasis and its implications for various diseases, including cancer and metabolic disorders. Recent studies have shown that ACSS2 expression is often upregulated in tumor cells, contributing to the increased demand for acetyl-CoA in rapidly proliferating tissues. This makes ACSS2 a potential biomarker for cancer and a target for therapeutic interventions. Furthermore, understanding the regulatory mechanisms and functional properties of ACSS2 can provide insights into metabolic reprogramming in cancer, as well as potential applications in metabolic engineering and synthetic biology. Consequently, investigating the recombinant expression of ACSS2 protein allows for detailed structural and functional analyses that could elucidate its enzymatic mechanisms, interactions with metabolites, and regulatory pathways, offering a pathway to novel therapeutic strategies in targeting metabolic aberrations. Such studies have the potential to significantly advance our understanding of cellular metabolism and its implications for health and disease.
