Analytical Data
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基因名
AARS1
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简介
The AARS1 protein facilitates the two-step process of attaching alanine to tRNA(Ala): first, alanine is activated by ATP to form Ala-AMP, and then it is transferred to the acceptor end of tRNA(Ala). AARS1 also corrects incorrectly charged tRNA(Ala) through its editing domain. AARS1 Protein, Mouse (sf9, His) is the recombinant mouse-derived AARS1 protein, expressed by Sf9 insect cells , with C-His labeled tag.
- Application
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别名
Alanine--tRNA ligase, cytoplasmic; Alanyl-tRNA synthetase; AlaRS; Sti; Aars
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种属
Mouse
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表达系统
Baculovirus
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标签
C-His
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q8BGQ7
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表达区间
M1-N968
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氨基酸序列
MDATLTAREIRERFINFFRRNEHTYVHSSATIPLDDPTLLFANAGMNQFKPIFLNTIDPSHPMAKLSRAANTQKCIRAGGKHNDLDDVGKDVYHHTFFEMLGSWSFGDYFKELACKMALELLTQEFGIPVERLYVTYFGGDEAAGLEPDLECRQIWQNLGLDEARILPGNMKDNFWEMGDTGPCGPCSEIHYDRIGGRDAAHLVNQDDPNVLEIWNLVFIQYNRESDGVLKPLPKKSIDTGMGLERLVSVLQNKMSNYDTDLFMPYFEAIQKGTGARPYTGKVGAEDADGIDMAYRVLADHARTITVALADGGRPDNTGRGYVLRRILRRAVRYSHEKLNASRGFFATLVDVVVQSLGDAFPELKKDPEMVKDIINEEEVQFLKTLSRGRRILDRKIQSLGDCKTIPGDTAWLLYDTYGFPVDLTGLIAEEKGLVVDMNGFEEERRLAQLKSQGKGAGDEDLIMLDIYAIEELRAKGLEATDDSPKYNYQSDSSGSYVFECTVATVLALRREKMFVDEVVTGQECGVVLDKTCFYAEQGGQIYDEGYLVKVDDSSEDKTEFTVKNAQVRGGYVLHIGTIYGNLKVGDQVRLFIDEPRRRPVMSNHTATHILNFALRSVLGEADQKGSLVAPDRLRFDFTAKGAMSTQQIKKAEEIVNGMIEAAKPVYTQDCPLAAAKAIQGLRAVFDETYPDPVRVVSIGVPVSELLDDPCGPAGSLTSVEFCGGTHLRNSSHAGAFVIVTEEAIAKGIRRIVAVTGAEAQKALRKSETLKKSLSAMEAKVKAQTAPNKDVQREIADLGEALATAVIPQWQKDEQRETLKSLKKVMDDLDRASKADVQKRVLEKTKQLIDSNPNQPLVILEMESGASAKALNEALKLFKTHSPQTSAMLFTVDNEAGKITCLCQVPQNAANRGLKASEWVQQVSGLMDGKGGGKDMSAQATGKNVGCLQEALQLATSFAQLRLGDVKN
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蛋白长度
Full Length
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分子量
108 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
AARS1, or Alanyl-tRNA synthetase 1, is an essential enzyme that catalyzes the attachment of alanine to its corresponding tRNA, playing a crucial role in protein synthesis. Beyond its fundamental function in translation, AARS1 is implicated in various cellular processes, including mitochondrial function and stress response. Recent studies have indicated that aberrations in AARS1 expression and function are associated with certain diseases, particularly in cancer and neurodegenerative disorders, making it a protein of significant interest for therapeutic targeting. The study of recombinant AARS1 proteins is essential for understanding its structure-function relationship and delineating the molecular mechanisms underlying its roles in health and disease. Researchers employ recombinant DNA technology to produce AARS1 with specific tags or mutations, facilitating biochemical assays and structural analyses. This biochemical exploration not only augments our understanding of the enzyme's catalytic mechanism but also lays the groundwork for drug development aimed at correcting the dysfunction associated with AARS1. Given the enzyme's pivotal role in cellular metabolism and its potential as a therapeutic target, ongoing research into recombinant AARS1 seeks to unveil insights that may contribute to innovative treatment strategies for conditions linked to protein misfolding and impaired translation processes.
