Analytical Data
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Gene name
ASS1
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简介
ASS1 Protein, Human (His) is a recombinant human Argininosuccinate synthase expressed in E. coli with a His tag. Argininosuccinate synthase is an enzyme that catalyzes the synthesis of argininosuccinate from citrulline and aspartate[1].
- Application
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Alternative Names
rHuASS1, His; Argininosuccinate Synthase; Citrulline--Aspartate Ligase; ASS1; ASS
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Species
Human
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Source
E. coli
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Tag
N-6*His
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Purity
Greater than 95% as determined by SDS-PAGE.
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Uniprot
P00966
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Expression Region
M1-K412
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AA Sequence
HHHHHHMSSKGSVVLAYSGGLDTSCILVWLKEQGYDVIAYLANIGQKEDFEEARKKALKLGAKKVFIEDVSREFVEEFIWPAIQSSALYEDRYLLGTSLARPCIARKQVEIAQREGAKYVSHGATGKGNDQVRFELSCYSLAPQIKVIAPWRMPEFYNRFKGRNDLMEYAKQHGIPIPVTPKNPWSMDENLMHISYEAGILENPKNQAPPGLYTKTQDPAKAPNTPDILEIEFKKGVPVKVTNVKDGTTHQTSLELFMYLNEVAGKHGVGRIDIVENRFIGMKSRGIYETPAGTILYHAHLDIEAFTMDREVRKIKQGLGLKFAELVYTGFWHSPECEFVRHCIAKSQERVEGKVQVSVLKGQVYILGRESPLSLYNEELVSMNVQGDYEPTDATGFININSLRLKEYHRLQSKVTAK
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Protein Length
Full Length
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Molecular Weight
50.0 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
ASS1 (argininosuccinate synthase 1) is an essential enzyme in the urea cycle, playing a critical role in the conversion of citrulline and aspartate into argininosuccinate, which is subsequently catabolized to produce arginine and fumarate. Mutations in the ASS1 gene can lead to argininosuccinic aciduria, a rare metabolic disorder characterized by hyperammonemia and various neurological impairments. Given its importance in metabolism and the implications of ASS1-related disorders, research on recombinant ASS1 protein has garnered significant attention. The recombinant expression of ASS1 allows for the detailed study of its enzymatic properties, structure-function relationships, and potential therapeutic applications. By producing large quantities of the protein, researchers can investigate the effects of specific mutations, identify potential inhibitors or activators, and explore its role in other physiological processes. Moreover, recombinant ASS1 can serve as a valuable tool in gene therapy approaches, aiming to restore normal metabolic function in affected individuals. This research ultimately contributes to our understanding of metabolic diseases and aids in the development of targeted treatments.
