Analytical Data
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Gene name
ACP5
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简介
The ACP5 protein may have a crucial role in bone resorption, particularly in the enzymatic activities involved in breaking down bone tissue. ACP5 is linked to the osteoclastic trap, which has higher affinity for nucleotide tri- and diphosphates. Understanding ACP5's mechanisms in bone resorption and its interactions with nucleotide substrates could offer insights into its role in maintaining bone health. ACP5 Protein, Mouse (HEK293, His) is the recombinant mouse-derived ACP5 protein, expressed by HEK293 , with C-His labeled tag.
- Application
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Alternative Names
Tartrate-resistant acid phosphatase type 5; TR-AP; TrATPase; T5ap; Trap
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Species
Mouse
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Source
HEK293
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Tag
C-His
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Purity
Greater than 95% as determined by SDS-PAGE.
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Uniprot
Q05117
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Expression Region
A23-P327
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AA Sequence
APTPTLRFVAVGDWGGVPNAPFHTAREMANAKEIARTVQTMGADFIMSLGDNFYFTGVHDASDKRFQETFEDVFSDRALRNIPWYVLAGNHDHLGNVSAQIAYSKISKRWNFPSPYYRLRFKIPRTNITVAIFMLDTVMLCGNSDDFASQQPKMPRDLGVARTQLSWLKKQLAAAKEDYVLVAGHYPIWSIAEHGPTRCLVKNLRPLLATYGVTAYLCGHDHNLQYLQDENGVGYVLSGAGNFMDPSVRHQRKVPNGYLRFHYGSEDSLGGFTHVEISPKEMTIIYVEASGKSLFKTSLPRRPRP
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Protein Length
Full Length of Mature Protein
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Molecular Weight
35.8-38 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
ACP5, also known as Acidic Calcium Phosphate Phosphatase 5, is a key enzyme involved in bone mineralization and phosphate metabolism. Its primary role is to hydrolyze inorganic pyrophosphate (PPi) to inorganic phosphate (Pi), which is essential for the proper mineralization of bone and teeth. Dysregulation of ACP5 activity can lead to pathological conditions such as osteomalacia and deficiencies in bone density, highlighting its significance in skeletal health. Recent studies have focused on the structure-function relationship of ACP5, utilizing recombinant protein techniques to produce and characterize the enzyme in vitro. This allows researchers to explore its catalytic mechanisms and regulatory pathways, providing insights into its role in bone physiology and potential therapeutic targets for bone-related diseases. Furthermore, understanding ACP5's interaction with other biomolecules and its regulation by post-translational modifications is crucial for elucidating its broader implications in metabolic disorders. The development of specific inhibitors or enhancers of ACP5 could pave the way for novel strategies in treating conditions associated with abnormal phosphate metabolism, illustrating the need for continued research in this area.
