Analytical Data
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基因名
GLA/alpha-Galactosidase A
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简介
GLA/alpha-Galactosidase A Protein is responsible for the breakdown of alpha-galactoside in the lysosome. GLA/alpha-Galactosidase A Protein participates in the negative regulation of nitric oxide biosynthesis and nitric oxide synthase activity. A deficiency of GLA/alpha-Galactosidase A Protein causes Fabry disease. GLA/alpha-Galactosidase A Protein, Mouse (HEK293, His) is the recombinant mouse-derived GLA/alpha-Galactosidase A protein, expressed by HEK293 , with C-His labeled tag.
- Application
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别名
Alpha-Galactosidase A; GALA; GLA; Melibiase
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种属
Mouse
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表达系统
HEK293
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标签
C-His
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纯度
Greater than 95% as determined by SDS-PAGE.
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蛋白编号
Q8BGZ6
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表达区间
L34-R421
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氨基酸序列
LDNGLARTPTMGWLHWERFMCNLDCQEEPDACISEQLFMQMAELMVSDGWRDAGYDYLCIDDCWMAPERDSKGRLQADPQRFPSGIKHLANYVHSKGLKLGIYADVGNKTCAGFPGSFGSYDIDAQTFADWGVDLLKFDGCHCDSVVSLENGYKYMALALNRTGRSIVYSCEWPLYLRPFHKPNYTDIQYYCNHWRNFDDVYDSWESIKNILSWTVVYQKEIVEVAGPGSWNDPDMLVIGNFGLSWDQQVTQMALWAIMAAPLLMSNDLRQISSQAKALLQNKDVIAINQDPLGKQGYCFRKENHIEVWERPLSNLAWAVAVRNLQEIGGPCPYTIQISSLGRGLACNPGCIITQLLPEKVHLGFYEWTLTLKTRVNPSGTVLFRLER
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蛋白长度
Full Length of Mature Protein (with Propeptide)
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分子量
46-52 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Alpha-galactosidase A (GLA) is a critical enzyme involved in the catabolism of glycosphingolipids, particularly in the breakdown of globotriaosylceramide (Gb3). Deficiency in GLA leads to Fabry disease, a rare genetic lysosomal storage disorder characterized by the accumulation of Gb3 in various tissues, resulting in a range of symptoms including renal impairment, cardiovascular issues, and neuropathic pain. Significant advancements in recombinant protein technology have enabled the production of GLA as a therapeutic agent. Recombinant GLA offers a promising approach for enzyme replacement therapy (ERT) in Fabry disease, addressing the underlying enzyme deficiency. Research into the optimal expression systems, purification methods, and enzymatic activity of recombinant GLA has been pivotal in enhancing its therapeutic efficacy and patient outcomes. Furthermore, ongoing studies are aimed at improving GLA stability, targeting, and bioavailability to maximize its clinical benefits. Understanding the structure and function of recombinant GLA, along with the development of novel delivery methods, holds the potential to revolutionize treatment strategies for Fabry disease, providing hope for affected individuals and families.
