Analytical Data
-
Gene name
CES2/carboxylesterase 2
-
简介
The CES2 protein plays a crucial role in detoxifying xenobiotics and activating ester and amide prodrugs. It has high catalytic efficiency, especially in the hydrolysis of substances such as cocaine, 4-methylumbelliferyl acetate, heroin and 6-monoacetylmorphine. CES2/carboxylesterase 2 Protein, Human (HEK293,C-His) is the recombinant human-derived CES2/carboxylesterase 2 protein, expressed by HEK293 , with C-His labeled tag.
- Application
-
Alternative Names
Cocaine esterase; Carboxylesterase 2; CE-2; CES2; ICE
-
Species
Human
-
Source
HEK293
-
Tag
C-His
-
Purity
Greater than 90% as determined by SDS-PAGE.
-
Uniprot
O00748-1
-
Expression Region
Q27-L559
-
AA Sequence
QDSASPIRTTHTGQVLGSLVHVKGANAGVQTFLGIPFAKPPLGPLRFAPPEPPESWSGVRDGTTHPAMCLQDLTAVESEFLSQFNMTFPSDSMSEDCLYLSIYTPAHSHEGSNLPVMVWIHGGALVFGMASLYDGSMLAALENVVVVIIQYRLGVLGFFSTGDKHATGNWGYLDQVAALRWVQQNIAHFGGNPDRVTIFGESAGGTSVSSLVVSPISQGLFHGAIMESGVALLPGLIASSADVISTVVANLSACDQVDSEALVGCLRGKSKEEILAINKPFKMIPGVVDGVFLPRHPQELLASADFQPVPSIVGVNNNEFGWLIPKVMRIYDTQKEMDREASQAALQKMLTLLMLPPTFGDLLREEYIGDNGDPQTLQAQFQEMMADSMFVIPALQVAHFQCSRAPVYFYEFQHQPSWLKNIRPPHMKADHGDELPFVFRSFFGGNYIKFTEEEEQLSRKMMKYWANFARNGNPNGEGLPHWPLFDQEEQYLQLNLQPAVGRALKAHRLQFWKKALPQKIQELEEPEERHTEL
-
Protein Length
Full Length of Isoform-1 Mature Protein
-
Molecular Weight
60.4 kDa.
-
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
-
Form
Freeze-dried powder
-
Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
-
Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
Carboxylesterase 2 (CES2) is a crucial enzyme involved in the hydrolysis of various esters, amides, and prodrugs, playing a significant role in drug metabolism and detoxification processes. Its importance is underscored by its involvement in the pharmacokinetics of numerous therapeutic agents, including angiotensin-converting enzyme inhibitors and anti-cancer drugs. Given the variability in CES2 activity among individuals due to genetic polymorphisms and environmental factors, research into CES2 is essential for understanding inter-individual differences in drug efficacy and toxicity. Recombinant CES2 protein production has become a focal point in enzymology, pharmacology, and drug development, facilitating in vitro studies that elucidate the enzyme's substrate specificity, catalytic mechanisms, and potential for generating drug-drug interactions. Moreover, the engineering of CES2 through recombinant technology has opened avenues for enhancing its enzymatic properties, aiding in the development of tailored therapeutics with improved profiles. Understanding CES2’s structure-function relationship is pivotal for the design of new drugs and optimizing existing ones, making it a critical target for both academic research and pharmaceutical applications.
