Analytical Data
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Gene name
IL-1 alpha
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简介
IL-1 alpha, found in non-hematopoietic cells, connects innate and adaptive immunity. Binding to IL1R1, it activates signaling pathways involving MYD88, IRAK1, and IRAK4, leading to NF-kappa-B and MAPK pathway activation. Released during cell death, IL-1 alpha induces inflammation, senses DNA damage, and interacts with TMED10, IL1R1, and S100A13 for secretion and plasma membrane translocation. IL-1 alpha Protein, Cynomolgus (HEK293, His) is the recombinant cynomolgus-derived IL-1 alpha protein, expressed by HEK293 , with C-His labeled tag.
- Application
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Alternative Names
Interleukin-1 alpha; IL-1 alpha; Hematopoietin-1; IL1A; IL1F1; IL-1 ALPHA; IL1α; IL-1A; IL1
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Species
Cynomolgus
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Source
HEK293
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Tag
C-8*His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P79340
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Expression Region
S113-A271
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Protein Length
Full Length of Mature Protein
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Molecular Weight
25-35 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Interleukin-1 alpha (IL-1α) is a pro-inflammatory cytokine that plays a crucial role in the regulation of immune responses, inflammation, and hematopoiesis. It is primarily produced by activated macrophages, but can also be expressed by various cell types, including endothelial cells and fibroblasts. IL-1α is involved in numerous physiological and pathological processes, including infection, tissue repair, and autoimmune diseases. Its activity is facilitated through binding to the IL-1 receptor, leading to the activation of signaling pathways that promote the expression of other pro-inflammatory cytokines, chemokines, and adhesion molecules. Given its central role in mediating inflammatory responses, IL-1α has become a significant target for therapeutic intervention in diseases characterized by chronic inflammation, such as rheumatoid arthritis, inflammatory bowel disease, and atherosclerosis. Recombinant IL-1α proteins are essential for studying its biological functions, understanding downstream signaling pathways, and developing IL-1α antagonists or inhibitors for potential clinical applications. Research involving recombinant IL-1α contributes to a deeper understanding of its intricate role in inflammation and immunity, and offers insights into novel therapeutic strategies aimed at modulating immune responses and treating inflammatory disorders.
