Analytical Data
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基因名
DCT
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简介
DCT (Dopachrome tautomerase) is pivotal in melanin biosynthesis, catalyzing the conversion of L-dopachrome to 5,6-dihydroxyindole-2-carboxylic acid (DHICA), a critical step in melanin synthesis. DCT's enzymatic activity is integral to the complex processes governing melanin production, emphasizing its role in skin pigmentation and the regulation of melanin-related functions. DCT Protein, Mouse (His-SUMO) is the recombinant mouse-derived DCT protein, expressed by E. coli , with N-10*His, N-SUMO labeled tag.
- Application
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种属
Mouse
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表达系统
E. coli
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标签
N-10*His;N-SUMO
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P29812
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表达区间
Q24-S472
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氨基酸序列
QFPRVCMTLDGVLNKECCPPLGPEATNICGFLEGRGQCAEVQTDTRPWSGPYILRNQDDREQWPRKFFNRTCKCTGNFAGYNCGGCKFGWTGPDCNRKKPAILRRNIHSLTAQEREQFLGALDLAKKSIHPDYVITTQHWLGLLGPNGTQPQIANCSVYDFFVWLHYYSVRDTLLGPGRPYKAIDFSHQGPAFVTWHRYHLLWLERELQRLTGNESFALPYWNFATGKNECDVCTDELLGAARQDDPTLISRNSRFSTWEIVCDSLDDYNRRVTLCNGTYEGLLRRNKVGRNNEKLPTLKNVQDCLSLQKFDSPPFFQNSTFSFRNALEGFDKADGTLDSQVMNLHNLAHSFLNGTNALPHSAANDPVFVVLHSFTDAIFDEWLKRNNPSTDAWPQELAPIGHNRMYNMVPFFPPVTNEELFLTAEQLGYNYAVDLSEEEAPVWSTTLS
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蛋白长度
Lumenal Domain
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
DCT (Dopachrome tautomerase) recombinant protein has garnered significant interest in recent years due to its crucial role in the melanogenesis pathway and potential applications in therapeutic interventions. Melanogenesis, the biochemical process responsible for the production of melanin, affects not only skin pigmentation but also plays a vital role in protecting against UV radiation and oxidative stress. DCT, as an enzyme involved in the conversion of dopachrome to dopaquinone, is essential for efficient melanin synthesis. The recombinant production of DCT allows for large-scale availability of the protein, facilitating advanced studies into its structure-function relationships, regulatory mechanisms, and role in various skin disorders such as vitiligo, melanoma, and albinism. Additionally, understanding the biochemical properties of DCT can lead to innovations in cosmetic applications and potential therapeutic strategies for pigmentation disorders. The recombinant version of DCT can also serve as a valuable tool in drug development and testing, providing insights into melanocyte biology and the effects of various compounds on pigmentation. As research progresses, DCT recombinant protein may play an increasingly important role in the fields of dermatology, pharmacology, and cosmetic science.
