Analytical Data
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Gene name
PMM1
- Application
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Alternative Names
Sec53; PMMH-22; Brain Glucose-1,6-Bisphosphatase
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Species
Human
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Source
E. coli
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Tag
N-His
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Purity
Greater than 95% as determined by SDS-PAGE.
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Uniprot
Q92871
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Expression Region
Ala2~Ala262
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Molecular Weight
38kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
PMM1 (phosphomannomutase 1) is an essential enzyme involved in the biosynthesis of glycoproteins and glycolipids, playing a critical role in the conversion of mannose-6-phosphate to mannose-1-phosphate, a crucial step in the carbohydrate metabolism pathway. Mutations in the PMM1 gene are associated with congenital disorders such as Congenital Disorders of Glycosylation (CDG), specifically CDG-Ib, which can lead to various clinical manifestations, including developmental delays, liver dysfunction, and immune deficiencies. Research involving the recombinant expression of PMM1 protein allows for the detailed study of its enzymatic properties, substrate specificity, and the impact of mutations on its function. By utilizing recombinant DNA techniques, scientists can produce large quantities of the PMM1 protein for biochemical assays, structural analysis, and potential therapeutic applications. Understanding the structure and function of PMM1 at a molecular level may provide insights into the mechanisms of glycosylation disorders and facilitate the development of novel treatments or gene therapies aimed at correcting metabolic defects. The investigation of PMM1 not only advances our knowledge of glycosylation pathways but also highlights the importance of proper protein function in human health, making it a significant target for biomedical research.
