Analytical Data
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基因名
PPIH
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简介
The PPIH protein is a peptidyl prolyl cis-trans isomerase (PPIase) that catalyzes the isomerization of prolyl imide peptide bonds and may contribute to protein folding. It plays a crucial role in pre-mRNA splicing and contributes to the formation of the U4/U5/U6 tri-snRNP complex in spliceosome assembly. PPIH Protein, Human (His) is the recombinant human-derived PPIH protein, expressed by E. coli , with N-6*His labeled tag.
- Application
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别名
Peptidyl-Prolyl Cis-Trans Isomerase H; PPIase H; Rotamase H; Small Nuclear Ribonucleoprotein Particle-Specific Cyclophilin H; CypH; U-snRNP-Associated Cyclophilin SnuCyp-20; USA-CYP; PPIH; CYP20; CYPH
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种属
Human
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表达系统
E. coli
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标签
N-6*His
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
O43447-1
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表达区间
M1-M177
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蛋白长度
Full Length of Isoform-1
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分子量
24.0 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
PPIH, or peptidyl-prolyl isomerase H, is a crucial enzyme that plays a significant role in various cellular processes, including protein folding, trafficking, and signal transduction. Its isomerase activity facilitates the conversion of proline residues between cis and trans configurations, which is vital for the proper folding and function of many proteins. Research on PPIH has gained momentum due to its involvement in critical physiological functions and its potential implications in various diseases, including cancer and neurodegenerative disorders. Understanding the structure and function of PPIH can provide insights into its molecular mechanisms and how alterations in its activity may contribute to disease pathology. Recent studies have focused on the biochemical characterization of PPIH as a recombinant protein, enabling a more detailed exploration of its enzymatic properties and interactions with other cellular components. This research is expected to advance our understanding of PPIH's role in cellular homeostasis and may lead to the development of novel therapeutic strategies targeting PPIH in disease contexts. By exploring the recombinant expression and purification of PPIH, researchers aim to elucidate its functional significance, thus paving the way for potential applications in biotechnology and medicine.
