Analytical Data
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Gene name
CDK5-p25
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简介
CDK5 is a proline-directed serine/threonine protein kinase that critically regulates neuronal cell cycle, differentiation and potential apoptosis in neuronal diseases by preventing cell cycle re-entry. It interacts with numerous proteins involved in neuronal development and coordinates processes such as survival, migration, differentiation, axonal growth, synaptogenesis, and neurotransmission. CDK5-p25 Protein, Human (sf9, GST, His, Flag) is the recombinant human-derived CDK5-p25, expressed by Sf9 insect cells, with N-GST, N-His, N-Flag labeled tag.
- Application
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Alternative Names
CDK5; cyclin-dependent kinase 5; PSSALRE; TPKII catalytic subunit; protein kinase CDK5 splicing; cell division protein kinase 5; serine/threonine-protein kinase PSSALRE; tau protein kinase II catalytic subunit;
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Species
Human
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Source
Baculovirus
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Tag
N-GST;N-His;N-Flag
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q00535-1 (M1-P292)&Q15078
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Expression Region
Q00535-1 (M1-P292)&Q15078 (A99-307)
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
CDK5 (Cyclin-Dependent Kinase 5) is a kinase that plays a critical role in neuronal development and function. Unlike other cyclin-dependent kinases, CDK5 is uniquely activated by its regulatory subunits, p35 and p25. The p25 fragment is derived from the cleavage of p35 by proteolytic enzymes, particularly during neuronal stress, and its accumulation is associated with various neurodegenerative diseases, including Alzheimer's disease. In contrast to p35, the p25 form of CDK5 leads to aberrant kinase activity, which has been implicated in the hyperphosphorylation of tau protein—a hallmark of neurodegenerative processes. Research involving the recombinant expression of CDK5-p25 fusion proteins allows for the detailed study of its enzymatic properties, substrate interactions, and downstream signaling pathways. Moreover, understanding the structural and functional distinctions between CDK5 complexes formed with p35 and p25 could provide insights into therapeutic targets for the prevention and treatment of tauopathies and dementia. Given the increasing prevalence of neurodegenerative disorders worldwide, elucidating the regulation of CDK5-p25 is a significant area of research, with potential implications for developing interventions aimed at restoring normal kinase function in affected neuronal populations.
