Analytical Data
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Gene name
TMEM165
- Application
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Alternative Names
TMEM165; TPARL; Transmembrane protein 165; Transmembrane protein PT27; Transmembrane protein TPARL
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Species
Human
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Source
E. coli
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Tag
N-10*His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9HC07
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Expression Region
M1-F324
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AA Sequence
MAAAAPGNGRASAPRLLLLFLVPLLWAPAAVRAGPDEDLSHRNKEPPAPAQQLQPQPVAVQGPEPARVEKIFTPAAPVHTNKEDPATQTNLGFIHAFVAAISVIIVSELGDKTFFIAAIMAMRYNRLTVLAGAMLALGLMTCLSVLFGYATTVIPRVYTYYVSTVLFAIFGIRMLREGLKMSPDEGQEELEEVQAELKKKDEEFQRTKLLNGPGDVETGTSITVPQKKWLHFISPIFVQATLTFLAEWGDRSQLTTIVLAAREDPYGVAVGGTVGHCLCTGLAVIGGRMIAQKISVRTVTIIGGIVFLAFAFSALFISPDSGF
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Protein Length
Full length protein
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
TMEM165 (Transmembrane Protein 165) is an integral membrane protein primarily located in the endoplasmic reticulum (ER) and is implicated in the homeostasis of divalent metal ions such as manganese and calcium, as well as vesicular trafficking. Mutations in the TMEM165 gene have been linked to a rare genetic disorder known as "Congenital Disorders of Glycosylation" (CDG) type IIb, characterized by severe developmental anomalies and various metabolic dysfunctions. Given its significant role in cellular metal ion regulation and glycosylation processes, the study of TMEM165 is crucial for understanding the molecular mechanisms underlying CDG and other related disorders. Recombinant TMEM165 protein offers a valuable tool for elucidating the protein's structural properties, functional mechanisms, and interaction patterns with other cellular components. Furthermore, by using the reconstituted protein in various assays, researchers aim to investigate its role in metal ion transport and stress responses within the ER. Insights gained from studying TMEM165 could pave the way for developing therapeutic strategies for related metabolic disorders, highlighting the importance of this protein as a target for future biomedical research.
