Analytical Data
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Gene name
H-2K(B) InsB Complex
- Application
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Alternative Names
H-2Kd & B2M & InsB (LYLVCGERL)
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Species
Mouse
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Source
HEK293
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Tag
C-Avi;C-His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P01901 (G22-T305)&P61769
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Expression Region
P01901 (G22-T305)&P61769 (I21-M119);LYLVCGERL
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Molecular Weight
50-55 kDa and 17 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
The H-2K(B) InsB complex is a crucial subject of study in immunology, particularly in the context of T cell recognition and autoimmune disease. This protein complex, formed between the H-2K(B) MHC class I molecule and the insulin B chain (InsB), serves as a model to investigate how peptide presentation influences T cell activation and specificity. The study of this complex is particularly relevant for understanding Type 1 Diabetes (T1D), a condition characterized by the autoimmune destruction of insulin-producing beta cells in the pancreas. In T1D, specific T cell responses target the InsB peptide, and identifying how the H-2K(B) presents this peptide can provide insights into disease mechanisms and potential therapeutic strategies. Recombinant production of the H-2K(B) InsB complex allows for detailed structural and functional analyses, aiding in the elucidation of the interactions between MHC molecules and T cell receptors (TCRs). Additionally, studying variations in peptide presentation may reveal the genetic and environmental factors contributing to the development of autoimmunity, paving the way for targeted interventions. Overall, research into the H-2K(B) InsB complex is pivotal for advancing our understanding of immune responses and their implications in autoimmune diseases, particularly in devising new approaches for prevention and treatment.
