Analytical Data
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Gene name
CDK1-CCNA2
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简介
The CDK1/CDC2-cyclin-B complex is a key regulator in the cell cycle. It plays an important role in cell division and development and regulates a variety of cellular activities, including chromosome segregation, nuclear membrane rupture, cytokinesis, etc. The activity of CDK1 is regulated by a variety of phosphorylation and dephosphorylation, thereby controlling the progression of the cell cycle and the DNA repair process. CDK1-CCNA2 Protein, Human (sf9, GST, His) is the recombinant human-derived CDK1-CCNA2, expressed by Sf9 insect cells, with N-His, N-GST labeled tag.
- Application
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Species
Human
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Source
Baculovirus
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Tag
N-His;N-GST
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P06493-1 (M1-M297)&P20248
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Expression Region
P06493-1 (M1-M297)&P20248 (E174-L432)
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
CDK1 (Cyclin-dependent kinase 1) is a critical regulator of the cell cycle, particularly during the transition from G2 phase to mitosis. It forms a complex with cyclin A2 (CCNA2), which is essential for driving cells through these processes. The CDK1-CCNA2 complex phosphorylates various substrates, leading to chromatin condensation, spindle assembly, and other mitotic events. Aberrations in this pathway can result in uncontrolled cell proliferation and are often implicated in cancer progression. Hence, understanding the biochemical properties and regulatory mechanisms of the CDK1-CCNA2 complex is vital for elucidating cell cycle control and its dysregulation in oncogenesis. The recombinant expression of CDK1-CCNA2 allows for in-depth studies of their interactions and functionalities under in vitro settings. By producing this protein complex in a controlled environment, researchers can investigate its structure, enzyme kinetics, and the effects of specific inhibitors or mutations. This information is crucial for the development of targeted therapies aimed at cancers associated with CDK1 dysregulation. Given the homologous nature of some other cyclin-dependent kinases and their cyclins, studies of the CDK1-CCNA2 complex can provide insights that extend to other members of the CDK family, ultimately contributing to a broader understanding of cell cycle regulation and its implications in cancer biology.
