Analytical Data
-
Gene name
CDK1
-
简介
The CDK1/CDC2-cyclin-B complex is a key regulator in the cell cycle. It plays an important role in cell division and development and regulates a variety of cellular activities, including chromosome segregation, nuclear membrane rupture, cytokinesis, etc. The activity of CDK1 is regulated by a variety of phosphorylation and dephosphorylation, thereby controlling the progression of the cell cycle and the DNA repair process. CDK1 Protein, Human (sf9) is the recombinant human-derived CDK1, expressed by Sf9 insect cells, with tag-free. The total length of CDK1 Protein, Human (sf9) is 297 a.a..
- Application
-
Alternative Names
Cyclin-dependent kinase 1; CDK1; p34 protein kinase; CDC2; CDC28A; CDKN1; P34CDC2
-
Species
Human
-
Source
Baculovirus
-
Tag
Tag Free
-
Purity
Greater than 90% as determined by SDS-PAGE.
-
Uniprot
P06493
-
Expression Region
M1-M297
-
Protein Length
Full Length
-
Molecular Weight
34.2 kDa
-
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
-
Form
Freeze-dried powder
-
Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
-
Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
CDK1, or Cyclin-Dependent Kinase 1, is a crucial regulatory enzyme that plays a pivotal role in cell cycle progression, particularly in the transition from G2 to M phase during mitosis. Its activity is tightly regulated by the binding of cyclins, with CDK1-cyclin B complex being essential for the initiation of mitosis. Dysregulation of CDK1 activity can lead to various pathologies, including cancer, making it a significant target for therapeutic intervention. The study of CDK1 recombinant proteins has gained attention in recent years as researchers seek to better understand its structure and function in the cell cycle. By producing CDK1 in a recombinant form, scientists can investigate its biochemical properties, post-translational modifications, and interactions with other cellular proteins in vitro. This research provides insights into the molecular mechanisms governing cell division and the potential development of novel anticancer strategies that target CDK1. The ability to generate large quantities of pure, functional CDK1 protein facilitates high-throughput screening of inhibitors and elucidation of its role in cancer biology, paving the way for new diagnostic and therapeutic approaches. Understanding how CDK1 functions in different cellular contexts further emphasizes its importance not only in cell cycle regulation but also in broader cellular processes, such as DNA damage response and apoptosis. The exploration of CDK1 recombinant proteins thus holds promise for advancing our understanding of cell cycle control and its implications in diseases associated with cell proliferation.
