Analytical Data
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Gene name
HLA-E*01:03&B2M&CMV UL40(VMAPRTLLL) Monomer
- Application
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Alternative Names
HLA-E*0103 & B2M & CMV UL40 (VMAPRTLLL)
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Species
Human
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Source
HEK293
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Tag
C-Avi;C-His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P13747 (G22-I305)&P61769
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Expression Region
P13747 (G22-I305)&P61769 (I21-M119)&VMAPRTLLL
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Molecular Weight
40-43 kDa and 12 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
HLA-E*01:03 is a non-classical major histocompatibility complex (MHC) Class I molecule that plays a crucial role in immune regulation and response, particularly in the context of viral infections. It is known to present the peptide UL40 derived from cytomegalovirus (CMV), specifically the sequence VMAPRTLLL, to immune cells, which helps in the modulation of natural killer (NK) cell activities. Due to its unique ability to interact with both NK cell receptors and T cells, HLA-E is an important target for studying immune evasion mechanisms employed by viruses. Understanding the structure and function of HLA-E*01:03 in conjunction with the B2M (beta-2-microglobulin) protein, which is essential for the stability and surface expression of MHC Class I molecules, can provide insights into the interplay between the host immune system and viral pathogens. The investigation of recombinant monomeric forms of HLA-E*01:03 in complex with B2M and the CMV UL40 peptide enables researchers to explore binding affinities, structural conformations, and potential therapeutic implications in vaccine design or immunotherapy strategies aimed at enhancing immune responses against CMV and other viral diseases. This research not only advances the understanding of the human immune system but also has significant implications for developing novel therapeutic approaches to combat viral infections and regulate immune tolerance in various clinical scenarios.
