Analytical Data
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Gene name
CHEK2
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简介
CHEK2 is a serine/threonine protein kinase that coordinates cellular responses to DNA double-strand breaks by activating cell cycle arrest, DNA repair, and apoptosis. It phosphorylates effectors and inhibits CDC25A, CDC25B, and CDC25C, thereby enhancing CDK cyclin inhibition and cell cycle arrest. CHEK2 Protein, Mouse (sf9, His-GST) is the recombinant mouse-derived CHEK2 protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag.
- Application
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Alternative Names
Serine/threonine-protein kinase Chk2; Hucds1; Checkpoint kinase 2; CDS1; CHK2; RAD53
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Species
Mouse
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Source
Baculovirus
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Tag
N-His;N-GST
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9Z265
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Expression Region
M1-L546
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Protein Length
Full Length
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Molecular Weight
90 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
CHEK2, or checkpoint kinase 2, is a serine/threonine kinase that plays a crucial role in DNA damage response and cell cycle regulation, particularly in maintaining genomic stability. Mutations in the CHEK2 gene have been linked to an increased risk of various cancers, including breast, prostate, and colon cancers. The study of recombinant CHEK2 protein has garnered significant attention as it allows researchers to explore the protein's structural and functional properties in detail, shedding light on its role in cancer biology. By expressing and purifying CHEK2 as a recombinant protein, scientists can investigate its kinase activity, interaction with other proteins involved in the DNA damage response, and its mechanistic role in tumorigenesis. Understanding the biochemical pathways in which CHEK2 is involved could reveal potential therapeutic targets for cancer treatment, particularly for tumors exhibiting CHEK2 mutations. Additionally, studying the post-translational modifications and functional domains of CHEK2 can enhance our comprehension of its regulatory mechanisms and susceptibility to oncogenic transformations. This research not only contributes to our fundamental understanding of cancer biology but also has implications for developing targeted therapies and improving cancer diagnosis based on genetic predispositions associated with CHEK2 variations.
