Analytical Data
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Gene name
Chk1
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简介
Chk1 is a serine/threonine protein kinase that critically coordinates checkpoint-mediated cell cycle arrest and DNA repair in response to DNA damage or unreplicated DNA. It negatively regulates unperturbed cell cycle progression and protects genome integrity by phosphorylating key targets such as CDC25A, CDC25B, CDC25C, RAD51, TP53, histone H3.1, RB1, SPRTN, TLK1 and FANCE. Chk1 Protein, Mouse (sf9, His-GST) is the recombinant mouse-derived Chk1 protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag.
- Application
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Alternative Names
Serine/threonine-protein kinase Chk1; Chek1; Chk1
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Species
Mouse
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Source
Baculovirus
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Tag
N-His;N-GST
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
O35280
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Expression Region
M1-T476
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Protein Length
Full Length
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Molecular Weight
78 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
CHK1 (Checkpoint Kinase 1) is a pivotal serine/threonine protein kinase that plays a crucial role in the cellular response to DNA damage and the regulation of the cell cycle. Its primary function is to maintain genomic stability by orchestrating the DNA damage response (DDR) pathways, particularly in the S and G2 phases of the cell cycle. Dysregulation of CHK1 has been implicated in various cancers, making it an attractive target for therapeutic interventions. Research into CHK1 recombinant proteins has gained momentum as they can be utilized to better understand its biochemical properties, signaling pathways, and interactions with other cellular proteins. Additionally, CHK1 inhibitors have emerged as promising cancer treatments, particularly in combination with other therapies such as chemotherapy and radiation, with the aim of enhancing the efficacy of these treatments by altering tumor cell responses. The production and characterization of CHK1 recombinant proteins enable scientists to dissect its functional mechanisms, investigate the structural basis for its interactions, and explore potential avenues for drug development. Given the critical role of CHK1 in cancer biology and therapeutic response, ongoing research seeks to elucidate its intricate network of regulation and establish a foundation for the application of CHK1-targeted therapies in clinical settings. This emerging field not only provides insights into cancer pathophysiology but also opens up new opportunities for innovative treatment strategies aimed at improving patient outcomes.
