Analytical Data
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Gene name
LOK
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简介
LOK is an important serine/threonine protein kinase that significantly regulates lymphocyte migration by phosphorylating MSN and potentially PLK1. It acts as a negative regulator of MAP3K1/MEKK1. LOK Protein, Human (His) is the recombinant human-derived LOK protein, expressed by E. coli , with N-His labeled tag.
- Application
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Alternative Names
Serine/threonine-protein kinase 10; STK10; LOK
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Species
Human
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Source
E. coli
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Tag
N-His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
O94804
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Expression Region
R18-E317
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Protein Length
Partial
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Molecular Weight
40 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
The LOK (Lymphokine-activated killer T-cell-originated protein kinase) is a member of the ste20-like kinase family and plays a crucial role in various cellular processes, including cell proliferation, survival, and apoptosis. Its unique expression pattern in immune cells has attracted significant research attention, particularly in the context of cancer immunotherapy and autoimmune diseases. Studies have shown that LOK is involved in the regulation of T-cell functions, making it a potential target for enhancing immune responses against tumors. Additionally, abnormalities in LOK signaling pathways may contribute to the development of malignancies and autoimmune disorders. The investigation of LOK recombinant proteins can thus provide valuable insights into its functional roles, mechanisms of action, and potential therapeutic applications. By expressing and purifying LOK recombinant proteins, researchers aim to understand its structure-function relationships, identify interaction partners, and elucidate its regulatory pathways. This research not only contributes to our fundamental understanding of immune regulation but also paves the way for novel therapeutic strategies that harness the power of the immune system to fight cancer and other diseases. As the field evolves, the development of specific LOK inhibitors or enhancers could lead to promising advancements in targeted therapies, emphasizing the importance of continued research in this area.
