Analytical Data
-
Gene name
MAPK4
-
简介
MAPK4, an atypical MAPK protein, phosphorylates microtubule-associated protein 2 (MAP2) and MAPKAPK5, forming a complex with intricate phosphorylation events. Interaction with MAPKAPK5 leads to ERK3/MAPK6 phosphorylation at Ser-189, activating MAPKAPK5, which reciprocally phosphorylates ERK3/MAPK6. The specific cellular role of this interplay, while unclear, suggests ERK3's potential involvement in promoting entry into the cell cycle. MAPK4 Protein, Human (sf9, His, GST) is the recombinant human-derived MAPK4 protein, expressed by sf9 insect cells , with N-8*His, N-GST labeled tag.
- Application
-
Alternative Names
MAPK4; Mitogen-activated protein kinase 4; MAP kinase 4; MAPK 4; Extracellular signal-regulated kinase 4; ERK-4; MAP kinase isoform p63; p63-MAPK
-
Species
Human
-
Source
Baculovirus
-
Tag
N-8*His;N-GST
-
Purity
Greater than 90% as determined by SDS-PAGE.
-
Uniprot
P31152
-
Expression Region
A2-W587
-
Protein Length
Partial
-
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
-
Form
Freeze-dried powder
-
Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
-
Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
The MAPK4 (Mitogen-Activated Protein Kinase 4) is a critical member of the MAPK family, which plays a crucial role in various cellular processes, including cell proliferation, differentiation, and response to stress signals. Initially identified in plants, MAPK4 has garnered attention for its involvement in regulating plant immune responses and developmental processes. In mammals, while MAPK4's functions have been less well-characterized, emerging studies suggest its significant role in modulating immune responses and cellular signaling pathways. The recombination and expression of MAPK4 as a recombinant protein have become essential for elucidating its biological functions, offering insights into its potential applications in improving plant disease resistance and understanding its signaling mechanisms in higher organisms. By generating and characterizing MAPK4 recombinant protein, researchers aim to explore its interaction with various substrates and signaling partners, contributing to a deeper understanding of MAPK signaling dynamics and its implications in health and disease. Additionally, recombinant MAPK4 can serve as a valuable tool in drug discovery and therapeutic interventions targeting MAPK pathways, emphasizing the need for extensive research on this intriguing protein.
