Analytical Data
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Gene name
Activin RIB/ALK-4
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简介
Activin RIB, also known as activin receptor type-1B (ACVR1B) or ALK-4, is a type I transmembrane serine/threonine kinase receptor that is part of the TGF-β receptor superfamily. Activin binds to a type II activin receptor (ACVR2or ACVR2B) and then recruits ACVR1B[1][2][3]. Activin RIB/ALK-4 Protein, Rhesus Macaque (HEK293, Fc) is produced in HEK293 cells with a C-Terminal Fc-tag. It consists of 100 amino acids (R27-E126).
- Application
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Biological Activity
Measured by its binding ability in a functional ELISA. When Recombinant Rhesus Macaque ALK-4/Activin RIB Protein is immobilized at 2µg/mL (100 µL/well) can bind Recombinant Human TDGF1. The ED50 for this effect is 18.31 ng/mL. Measured by its binding ability in a functional ELISA. When Recombinant Rhesus Macaque ALK-4/Activin RIB Protein is immobilized at 2 µg/mL (100 µL/well) can bind Recombinant Human TDGF1. The ED50 for this effect is 18.31 ng/mL.
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Alternative Names
Activin Receptor IB; ALK-4; Activin RIB; ACVR1B
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Species
Rhesus Macaque
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Source
HEK293
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Tag
C-hFc
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Purity
Greater than 95% as determined by SDS-PAGE.
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Uniprot
I2CYX8
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Expression Region
R27-E126
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Protein Length
Partial
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Molecular Weight
43-45 kDa.
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Activin RIB, also known as ALK-4, is a type I receptor that plays a critical role in the Activin signaling pathway, which is involved in various biological processes, including embryonic development, cell differentiation, and tissue homeostasis. The study of Activin RIB/ALK-4 recombinant proteins has garnered significant interest due to their implications in disease mechanisms, particularly in cancer, fibrosis, and reproductive health. Activin signaling is known to regulate cell proliferation and apoptosis, and dysregulation of this pathway can lead to pathological conditions. Additionally, Activin RIB/ALK-4 interacts with other members of the TGF-β superfamily, suggesting its involvement in complex molecular networks. Recombinant protein studies facilitate a deeper understanding of receptor-ligand interactions and downstream signaling cascades, which can aid in the identification of potential therapeutic targets. Furthermore, the use of recombinant proteins allows for the exploration of the physiological roles of Activin RIB in various cell types and contexts, setting the stage for the development of novel strategies for manipulating this pathway in disease treatment and regenerative medicine. The ongoing research on Activin RIB/ALK-4 promises to illuminate its functional significance and offer insight into potential clinical applications.
