Analytical Data
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基因名
p53
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简介
The p53 protein acts as a tumor suppressor, regulates the cell cycle and induces growth arrest or apoptosis.It activates genes that inhibit cell division and trigger apoptosis by controlling the expression of various proteins.p53 Protein, Rat (His) is the recombinant rat-derived p53 protein, expressed by E.coli , with N-6*His labeled tag.
- Application
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别名
Tp53; P53; Cellular tumor antigen p53; Tumor suppressor p53
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种属
Rat
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表达系统
E. coli
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标签
N-6*His
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P10361
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表达区间
M1-D391
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氨基酸序列
MEDSQSDMSIELPLSQETFSCLWKLLPPDDILPTTATGSPNSMEDLFLPQDVAELLEGPEEALQVSAPAAQEPGTEAPAPVAPASATPWPLSSSVPSQKTYQGNYGFHLGFLQSGTAKSVMCTYSISLNKLFCQLAKTCPVQLWVTSTPPPGTRVRAMAIYKKSQHMTEVVRRCPHHERCSDGDGLAPPQHLIRVEGNPYAEYLDDRQTFRHSVVVPYEPPEVGSDYTTIHYKYMCNSSCMGGMNRRPILTIITLEDSSGNLLGRDSFEVRVCACPGRDRRTEEENFRKKEEHCPELPPGSAKRALPTSTSSSPQQKKKPLDGEYFTLKIRGRERFEMFRELNEALELKDARAAEESGDSRAHSSYPKTKKGQSTSRHKKPMIKKVGPDSD
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蛋白长度
Full Length
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分子量
50 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
The p53 protein, often referred to as the "guardian of the genome," plays a crucial role in regulating the cell cycle and maintaining genomic stability. It is encoded by the TP53 gene and acts as a tumor suppressor, preventing the proliferation of cells with damaged DNA. Mutations in the TP53 gene are found in approximately half of all human cancers, underscoring its significance in cancer biology. Research into p53 has gained momentum due to its pivotal role in regulating apoptosis, DNA repair, and cellular senescence. Recombinant p53 protein has been developed for various applications, including studying its functional mechanisms, developing cancer therapies, and designing targeted treatments that restore the activity of mutated forms of p53. Furthermore, innovative techniques such as CRISPR/Cas9 genome editing are being explored to create cellular models that express mutated p53, enhancing our understanding of its role in tumorigenesis. The characterization and application of recombinant p53 allow researchers to elucidate its biochemical pathways and interactions with other cellular proteins, offering insights into potential therapeutic strategies to combat cancers associated with p53 dysfunction. Overall, p53 and its recombinant protein derivatives represent a vital area of research in oncology, with the potential to transform cancer treatment paradigms and improve patient outcomes.
