Analytical Data
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Gene name
TYRO3/DTK
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简介
The TYRO3/DTK protein is a tyrosine kinase receptor. TYRO3 transduces signals from the extracellular matrix by activating the PI3K/AKT/ NF-kappa-B pathway. TYRO3 plays an important role in inhibiting Toll-like receptor (TLRs) mediated innate immune response by activating STAT1. TYRO3 has the function of promoting tumor cell survival and/or proliferation, metastasis and chemotherapy resistance. TYRO3/DTK Protein, Human (Q261H, HEK293, Fc) is the recombinant human-derived TYRO3/DTK protein, expressed by HEK293 , with C-hFc labeled tag.
- Application
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Biological Activity
Immobilized TYRO3 at 2 μg/mL (100 μL/well) can bind Biotinylated GAS6 protein. The ED50 for this effect is 161.5 ng/mL, corresponding to a specific activity is 6.19×10^3 Unit/mg. Immobilized TYRO3 at 2 μg/mL (100 μL/well) can bind Biotinylated GAS6 protein. The ED50 for this effect is 161.5 ng/mL, corresponding to a specific activity is 6.19×103 Unit/mg.
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Alternative Names
TYRO3; BYK; DTK; RSE; SKY; TIF
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Species
Human
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Source
HEK293
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Tag
C-hFc
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Purity
Greater than 95% as determined by SDS-PAGE.
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Uniprot
Q06418
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Expression Region
A41-S428, Q261H
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AA Sequence
AGLKLMGAPVKLTVSQGQPVKLNCSVEGMEEPDIQWVKDGAVVQNLDQLYIPVSEQHWIGFLSLKSVERSDAGRYWCQVEDGGETEISQPVWLTVEGVPFFTVEPKDLAVPPNAPFQLSCEAVGPPEPVTIVWWRGTTKIGGPAPSPSVLNVTGVTQSTMFSCEAHNLKGLASSRTATVHLQALPAAPFNITVTKLSSSNASVAWMPGADGRALLQSCTVHVTQAPGGWEVLAVVVPVPPFTCLLRDLVPATNYSLRVRCANALGPSPYADWVPFQTKGLAPASAPQNLHAIRTDSGLILEWEEVIPEAPLEGPLGPYKLSWVQDNGTQDELTVEGTRANLTGWDPQKDLIVRVCVSNAVGCGPWSQPLVVSSHDRAGQQGPPHSRTS
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Protein Length
Extracellular Domain
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Molecular Weight
85-120 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
TYRO3/DTK is a member of the TAM receptor tyrosine kinase family, which includes AXL and MER. These receptors play crucial roles in various biological processes, including regulation of immune responses, tissue homeostasis, and clearance of apoptotic cells. The study of TYRO3/DTK has gained attention due to its involvement in cancer biology, where it may contribute to tumor progression and resistance to therapies. Dysregulation of TYRO3/DTK signaling has been linked to several malignancies, making it a potential target for therapeutic interventions. Furthermore, the receptor's role in modulating inflammatory responses suggests its importance in autoimmune diseases and other inflammatory conditions. Given these factors, the recombinant protein of TYRO3/DTK presents an opportunity to explore its functional roles in cellular signaling pathways and to develop potential therapeutic strategies for treating various diseases. Recombinant TYRO3/DTK can be used in assays to study its binding interactions, downstream signaling mechanisms, and effects on cell behavior, providing insights into its biological significance and potential as a drug target. Understanding the structural and functional aspects of TYRO3/DTK is essential for elucidating its mechanisms of action and for the development of novel treatments aimed at inhibiting its pathological functions. This research may pave the way for innovative therapeutic approaches that exploit the modulation of TYRO3/DTK signaling in disease management.
