Analytical Data
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基因名
ygiS
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简介
The ygiS protein may be an important component of the deoxycholate transport system and play an important role in cellular responses to bile acid detergents, especially deoxycholate. Its absence leads to increased levels of intracellular deoxycholic acid, resulting in decreased cell growth. ygiS Protein, E.coli (His-SUMO) is the recombinant E. coli-derived ygiS protein, expressed by E. coli , with N-His, N-SUMO labeled tag.
- Application
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别名
ygiS; b3020; JW2988; Probable deoxycholate-binding periplasmic protein YgiS
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种属
E.coli
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表达系统
E. coli
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标签
N-His;N-SUMO
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q46863
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表达区间
A21-H535
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氨基酸序列
ADVPANTPLAPQQVFRYNNHSDPGTLDPQKVEENTAAQIVLDLFEGLVWMDGEGQVQPAQAERWEILDGGKRYIFHLRSGLQWSDGQPLTAEDFVLGWQRAVDPKTASPFAGYLAQAHINNAAAIVAGKADVTSLGVKATDDRTLEVTLEQPVPWFTTMLAWPTLFPVPHHVIAKHGDSWSKPENMVYNGAFVLDQWVVNEKITARKNPKYRDAQHTVLQQVEYLALDNSVTGYNRYRAGEVDLTWVPAQQIPAIEKSLPGELRIIPRLNSEYYNFNLEKPPFNDVRVRRALYLTVDRQLIAQKVLGLRTPATTLTPPEVKGFSATTFDELQKPMSERVAMAKALLKQAGYDASHPLRFELFYNKYDLHEKTAIALSSEWKKWLGAQVTLRTMEWKTYLDARRAGDFMLSRQSWDATYNDASSFLNTLKSDSEENVGHWKNAQYDALLNQATQITDATKRNALYQQAEVIINQQAPLIPIYYQPLIKLLKPYVGGFPLHNPQDYVYSKELYIKAH
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蛋白长度
Full Length of Mature Protein
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
YgiS, a protein associated with various biological processes, has garnered attention in recent years due to its potential roles in bacterial physiology and virulence. Initially identified in certain pathogenic strains of bacteria, YgiS has been suggested to play a critical role in stress response and metabolic regulation. Researchers have been particularly interested in its structure-function relationships, as the protein appears to be involved in the modulation of signaling pathways essential for bacterial adaptation to environmental challenges. Investigations into the recombinant expression of YgiS have enabled scientists to explore its biochemical properties and interactions with other cellular components in detail. This research is crucial, as understanding YgiS may contribute to the development of novel therapeutic strategies against bacterial infections. The protein’s involvement in enzymatic activities and regulatory mechanisms positions it as a potential target for innovative drug design aimed at disrupting virulence factors in pathogenic microbes. Consequently, characterizing the YgiS recombinantly not only provides insights into its fundamental roles within microbial cells but also opens avenues for applied research in infectious disease management. As the field of protein engineering advances, efforts to elucidate the functional implications of YgiS could ultimately lead to breakthroughs in our understanding of microbial resilience and pathogenicity.
