Analytical Data
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基因名
DAPK1
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简介
The DAPK1 protein is a calcium/calmodulin-dependent kinase involved in multiple cellular pathways, regulating cell survival, apoptosis, and autophagy. The DAPK1 protein also acts as a signal amplifier for NMDA receptors, causing brain damage in stroke. DAPK1 Protein, Human (sf9, His-GST) is the recombinant human-derived DAPK1 protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag.
- Application
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别名
Death-associated protein kinase 1; DAP kinase 1; DAPK
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种属
Human
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表达系统
Baculovirus
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标签
N-His;N-GST
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P53355-1
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表达区间
M1-L363
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蛋白长度
Partial
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分子量
64 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
DAPK1 (Death-Associated Protein Kinase 1) is a serine/threonine kinase that plays a crucial role in various cellular processes, including apoptosis, autophagy, and immune responses. Its dysregulation has been implicated in numerous diseases, particularly cancer, where it can function both as a tumor suppressor and a promoter, depending on the context. Research into DAPK1 has gained momentum due to its potential as a therapeutic target and biomarker. The ability to produce recombinant DAPK1 protein has significantly advanced studies aimed at understanding its structure and function, as well as its interactions with other cellular molecules. This recombinant protein enables detailed investigations into its enzymatic activity, phosphorylation sites, and the mechanisms by which it regulates cell death and survival pathways. Furthermore, the availability of purified DAPK1 supports the development of high-throughput screening assays for small-molecule inhibitors or activators, which may lead to novel cancer therapies. Ongoing research into the biochemical properties and physiological roles of DAPK1 is critical to elucidating its function in health and disease, paving the way for innovative therapeutic strategies targeting this pivotal kinase.
