Analytical Data
-
Gene name
Semaphorin-3A/SEMA3A
- Application
-
Alternative Names
Semaphorin III; SEMA3A; SEMAD; Hsema-I; SEMA1; SemD; Semad; coll-1 ; Hsema-III; Sema III
-
Species
Human
-
Source
HEK293
-
Tag
C-hFc
-
Purity
Greater than 90% as determined by SDS-PAGE.
-
Uniprot
Q14563
-
Expression Region
K26-V771, R551A, R555A, R730A, K731A, R733A, R734A, R757A, R760A
-
Molecular Weight
120-135 kDa
-
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
-
Form
Freeze-dried powder
-
Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
-
Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
Semaphorin-3A (SEMA3A) is a member of the semaphorin family of proteins, which are known to play crucial roles in axon guidance, neuronal development, and immune responses. Initially identified for its function in the nervous system, SEMA3A acts as a chemorepellent, guiding developing neurons by signaling through its receptor neuropilin-1 and plexin-A1. Recent studies have expanded our understanding of SEMA3A's multifaceted roles beyond neural development, revealing its involvement in various physiological processes, including cardiovascular development, tumor suppression, and immune modulation. Dysregulation of SEMA3A is associated with several pathological conditions, such as neurodegenerative diseases, cancer metastasis, and autoimmune disorders, highlighting its potential as a therapeutic target. The recombinant production of SEMA3A protein allows for detailed studies of its functional mechanisms and interactions within the cellular microenvironment. This research not only aids in elucidating the biological roles of SEMA3A but also presents opportunities for developing novel therapeutic strategies that leverage SEMA3A's signaling pathways to address various diseases. Understanding the structure-function relationship of recombinant SEMA3A is essential for optimizing its application in both basic research and clinical settings, especially for diseases where SEMA3A's signaling may be misregulated. Overall, SEMA3A represents a promising area of study with significant implications for neurobiology, immunology, and cancer research.
