Analytical Data
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Gene name
RSPO3/R-spondin-3
- Application
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Alternative Names
R-spondin-3; RSPO3; Protein with TSP type-1 repeat; PWTSR; THSD2; CRISTIN1
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Species
Human
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Source
HEK293
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Tag
C-hFc
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9BXY4-1
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Expression Region
Q22-H272
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Molecular Weight
55-70 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
R-spondin-3 (RSPO3) is a member of the R-spondin family, which plays a critical role in modulating the Wnt signaling pathway, a key regulator of cellular processes such as proliferation, differentiation, and stem cell maintenance. RSPO proteins enhance Wnt signaling by stabilizing the interaction between Wnt ligands and their receptors, thereby promoting downstream signaling events. Research has shown that RSPO3 is involved in various biological processes, including development, tissue regeneration, and the maintenance of intestinal stem cells. Abnormal regulation of RSPO3 has been implicated in several diseases, including cancers and gastrointestinal disorders. Given its significance, the production and functional characterization of recombinant RSPO3 protein are essential for understanding its biological roles and therapeutic potential. This research provides valuable insights into the mechanisms underlying Wnt signaling modulation and paves the way for developing novel strategies for targeting RSPO3 in disease contexts. Continued studies may reveal how RSPO3 can be harnessed in regenerative medicine and cancer therapy, making it a promising target for future biomedical applications.
