Analytical Data
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Gene name
IAPP
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简介
The IAPP protein selectively inhibits insulin-stimulated glucose utilization and glycogen deposition in muscle, affecting glucose metabolism without affecting adipocytes. IAPP interacts with IDE (insulin-degrading enzyme) and insulin (INS) to form homodimers, affecting their fibril formation. IAPP Protein, Human (P. pastoris, His) is the recombinant human-derived IAPP protein, expressed by P. pastoris, with N-6*His labeled tag.
- Application
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Alternative Names
Amylin; DAP; Diabetes associated peptide; Diabetes-associated peptide; IAP; IAPP; IAPP_HUMAN; Insulinoma amyloid peptide; Islet amyloid polypeptide diabetes associated peptide, amylin; Islet amyloid polypeptide
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Species
Human
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Source
P. pastoris
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Tag
N-6*His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P10997
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Expression Region
K34-Y70
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Protein Length
Partial
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Molecular Weight
12 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
The research on IAPP (islet amyloid polypeptide) recombinant proteins stems from the growing recognition of their pivotal role in diabetes, particularly Type 2 diabetes mellitus. IAPP, co-secreted with insulin by beta cells in the pancreas, is known for its amyloidogenic properties, which lead to the formation of toxic aggregates associated with beta cell dysfunction and loss. Studies have demonstrated that misfolded IAPP contributes significantly to the pathogenesis of diabetes, making it a critical target for therapeutic interventions. The recombinant production of IAPP allows for a detailed exploration of its biophysical properties, mechanisms of aggregation, and interactions with cellular components. This research aims to unravel the molecular basis of IAPP-related pathologies, facilitate the development of potential inhibitors to mitigate amyloid formation, and enhance understanding of beta cell preservation strategies. Ultimately, advancing knowledge in this area may lead to novel treatment avenues for managing or preventing Type 2 diabetes and mitigating its complications.
