Analytical Data
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Gene name
POLD2
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简介
The POLD2 protein is a key component of DNA polymerase delta (Pol-delta) and DNA polymerase zeta (Pol-zeta) complexes, which are essential for genome replication fidelity and repair. In Pol-delta3 and Pol-delta4 forms, POLD2 contributes to high-fidelity lagging strand synthesis. POLD2 Protein, Human (His) is the recombinant human-derived POLD2, expressed by E. coli, with C-6*His labeled tag.
- Application
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Alternative Names
DNA Polymerase Delta Subunit 2; DNA Polymerase Delta Subunit p50; POLD2
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Species
Human
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Source
E. coli
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Tag
C-6*His
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Purity
Greater than 95% as determined by SDS-PAGE.
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Uniprot
P49005
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Expression Region
M1-P469
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AA Sequence
MFSEQAAQRAHTLLSPPSANNATFARVPVATYTNSSQPFRLGERSFSRQYAHIYATRLIQMRPFLENRAQQHWGSGVGVKKLCELQPEEKCCVVGTLFKAMPLQPSILREVSEEHNLLPQPPRSKYIHPDDELVLEDELQRIKLKGTIDVSKLVTGTVLAVFGSVRDDGKFLVEDYCFADLAPQKPAPPLDTDRFVLLVSGLGLGGGGGESLLGTQLLVDVVTGQLGDEGEQCSAAHVSRVILAGNLLSHSTQSRDSINKAKYLTKKTQAASVEAVKMLDEILLQLSASVPVDVMPGEFDPTNYTLPQQPLHPCMFPLATAYSTLQLVTNPYQATIDGVRFLGTSGQNVSDIFRYSSMEDHLEILEWTLRVRHISPTAPDTLGCYPFYKTDPFIFPECPHVYFCGNTPSFGSKIIRGPEDQTVLLVTVPDFSATQTACLVNLRSLACQPISFSGFGAEDDDLGGLGLGP
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Protein Length
Full Length
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Molecular Weight
53 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
RBP2 (Retinoblastoma Binding Protein 2) is a crucial enzyme that plays a significant role in histone demethylation, specifically in the removal of methyl groups from lysine residues on histone proteins. This process is vital for the regulation of gene expression, influencing various biological processes such as development, differentiation, and response to environmental stimuli. The dysregulation of RBP2 has been linked to several diseases, including cancer, where altered gene expression profiles contribute to tumor progression and metastasis. Additionally, RBP2 has been implicated in neurodegenerative diseases, highlighting its importance in maintaining cellular homeostasis. Given its central role in epigenetic regulation, recombinant RBP2 protein has garnered attention in the fields of molecular biology and therapeutics. Researchers are focusing on characterizing RBP2's enzymatic activity, understanding its interaction with other proteins, and exploring its potential as a target for drug development. By producing recombinant RBP2, scientists can investigate its biochemical properties, elucidate its functional mechanisms, and evaluate its role in disease pathways. Furthermore, recombinant protein studies have the potential to uncover new therapeutic strategies by identifying small molecule inhibitors or activators that can modulate RBP2 activity, thus offering promising avenues for treatment in cancer and other related disorders. Overall, the research on RBP2 and its recombinant protein form represents a crucial intersection of epigenetics, cancer biology, and therapeutic development, aiming to provide deeper insights into both fundamental biology and clinical applications.
