Analytical Data
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基因名
TrkA
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简介
TrkA Protein, Rabbit (HEK293, His) is a nerve growth factor (NGF) receptor that belongs to the tyrosine kinase receptor family. TRKA (NTRK1) gene encodes the high affinity receptor for a neurotrophin nerve growth factor (NGF). It is critical for the correct development of many types of neurons including pain-mediating sensory neurons and also controls proliferation, differentiation and survival of many neuronal and non-neuronal cells[1][2]. TrkA Protein, Rabbit (HEK293, His) is the recombinant Rabbit-derived TrkA protein, expressed by HEK293 , with C-His labeled tag.
- Application
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生物活性
Measured by its ability to inhibit NGF-induced proliferation of TF-1 human erythroleukemic cells. The ED50 for this effect is 0.9070 μg/mL in the presence of 10 ng/mL of NGF, corresponding to a specific activity is 1.103×103 U/mg Measured by its ability to inhibit NGF-induced proliferation of TF-1 human erythroleukemic cells. The ED50 for this effect is 0.9070 μg/mL in the presence of 10 ng/mL of NGF, corresponding to a specific activity is 1.103×103 U/mg
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别名
High affinity nerve growth factor receptor; Trk-A; NTRK1; MTC; TRK
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种属
Rabbit
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表达系统
HEK293
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标签
C-6*His
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纯度
Greater than 95% as determined by SDS-PAGE.
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蛋白编号
XP_008262512
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表达区间
A33-E414
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氨基酸序列
AALCPDVCCPRGPSGLLCTRPGALDRLRHLPGIENLTELYLENQNLQHLTLGDLRGLRELRNLAIVNSGLQSVATDAFRFTPRLSHLNLSFNALESLSWKTVQGLPLQELVLSGNSLRCSCALRWLQRWEEEGLAGVREQKLRCSESEPLALMPNASCGMPTLKVQMPNGSVDVGDSVFLQCQVEGQGLEKAGWSLTELEELATVMIQKSEDLPTLRLTLANVTSDLNRKNVTCWAENDVGRTEVSVQVNVSFPASVQLHTAVEMHHWCIPFSVDGQPAPSLHWLFNGSVLNETSFIFTEFLEPAANETMRHGCLRLNQPTHVNNGNYTLLATNPSGQAAASIMAAFMDNPFEFNPEDPIPVSFSPVDANSTSGDPVEKKDE
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蛋白长度
Partial
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分子量
45-100 kDa.
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
TrkA, also known as tropomyosin receptor kinase A, is a critical receptor tyrosine kinase that mediates the effects of nerve growth factor (NGF) and plays a vital role in neuronal survival, differentiation, and synaptic plasticity. Research on TrkA has gained significant traction due to its implications in neurodevelopmental and neurodegenerative disorders, as well as its potential as a therapeutic target in cancer. The recombination of TrkA proteins allows for the study of various isoforms and their functional roles in cellular signaling pathways. Recombinant TrkA proteins are produced through genetic engineering techniques, enabling researchers to explore the structure-function relationships of this receptor, its interaction with ligands, and the downstream signaling cascades activated by NGF binding. Additionally, TrkA reconstitution studies facilitate drug discovery efforts aimed at designing small molecules or biologics that can modulate TrkA activity, thereby offering potential therapeutic strategies for diseases associated with TrkA dysregulation. Understanding the dynamics of TrkA in health and disease continues to be a pivotal area of molecular neuroscience and pharmacology, further emphasizing the importance of TrkA recombinant protein studies in both basic and applied research contexts.
