Analytical Data
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基因名
PE-Labeled HLA-A*02:01&B2M&p53(G25-I308) Tetramer
- Application
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别名
HLA-A*0201 & B2M & p53 (HMTEVVRHC)
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种属
Human
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表达系统
HEK293
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标签
C-His;C-Avi
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P61769-1 (I21-M119)&AAA59606.1
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表达区间
P61769-1 (I21-M119)&AAA59606.1 (G25-I308)
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
The study of PE-labeled HLA-A*02:01/B2M/p53(G25-I308) tetramer recombinant proteins is fundamentally rooted in the exploration of cancer immunotherapy and the role of the immune system in recognizing and combating tumor cells. HLA-A*02:01 is one of the most prevalent human leukocyte antigen (HLA) class I molecules, critical for presenting peptide antigens to CD8+ T cells, thereby triggering immune responses. Mutations in the p53 tumor suppressor gene, particularly the G25-I308 variant, are commonly associated with various cancers, leading to abnormal cellular behavior and immune evasion. By generating a tetramer that includes this specific p53 peptide, researchers aim to monitor and quantify T cell responses against tumors harboring this mutation. The use of phycoerythrin (PE) labeling enhances detection during flow cytometry, allowing for precise analysis of T cell populations that are potentially reactive to p53-mutated cells. This research is significant for developing personalized cancer vaccines and improving immunotherapeutic strategies, ultimately enhancing patient outcomes in cancer treatment.
