Analytical Data
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Gene name
Apolipoprotein E/APOE2
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简介
Apolipoprotein E (APOE) plays a key role in lipoprotein-mediated lipid transport and is a core component in the production, transformation, and clearance of plasma lipoproteins. As an amphipathic molecule, APOE binds to various lipoprotein particles, including chylomicrons, chylomicron remnants, VLDL, and IDL, favoring HDL. Apolipoprotein E/APOE2 Protein, Human (C130R, HEK293) is the recombinant human-derived Apolipoprotein E/APOE2 protein, expressed by HEK293, with tag free.
- Application
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Alternative Names
Apolipoprotein E; Apo-E; APOE; apolipo E; APOE3
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Species
Human
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Source
HEK293
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Tag
Tag Free
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P02649-1
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Expression Region
K19-H317, C130R
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Protein Length
Full Length of Isoform-1 Mature Protein
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Molecular Weight
34-41 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Apolipoprotein E (ApoE) is a crucial protein involved in lipid metabolism, with three major isoforms: ApoE2, ApoE3, and ApoE4, which differ in their genetic sequence and functional properties. Among these, ApoE2 is notable for its potential protective effects against neurodegenerative diseases, particularly Alzheimer's disease. Research indicates that ApoE4 is associated with increased risk and earlier onset of Alzheimer's, while ApoE2 may confer a lower risk. This has sparked interest in studying the role of ApoE2 in neuroprotection and its mechanisms at the molecular level. Understanding the structure and function of recombinant ApoE2 protein can provide insights into its lipid-binding capabilities, interactions with neuronal cells, and effects on amyloid-beta metabolism. Investigating recombinant ApoE2 can also help establish its potential therapeutic applications, such as in developing strategies to modulate cholesterol levels and enhance neural repair processes. Overall, the study of recombinant ApoE2 protein is imperative for elucidating its role in neurodegeneration and exploring avenues for interventions that may leverage its beneficial properties.
