Cat: IPD-X29560

Recombinant Human MAT1A Protein,His

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Analytical Data

  • Gene name

    MAT1A

  • Application

    SPRMSTBLIITCELISACELL ASSAYDRUG SCREENING

  • Alternative Names

    AdoMet synthase 1; Methionine adenosyltransferase 1(MAT 1); Methionine adenosyltransferase I/III(MAT-I/III)

  • Species

    Human

  • Source

    E. coli

  • Tag

    N-10*His

  • Purity

    Greater than 90% as determined by SDS-PAGE.

  • Uniprot

    Q00266

  • Expression Region

    M1-F395

  • AA Sequence

    MNGPVDGLCDHSLSEGVFMFTSESVGEGHPDKICDQISDAVLDAHLKQDPNAKVACETVCKTGMVLLCGEITSMAMVDYQRVVRDTIKHIGYDDSAKGFDFKTCNVLVALEQQSPDIAQCVHLDRNEEDVGAGDQGLMFGYATDETEECMPLTIILAHKLNARMADLRRSGLLPWLRPDSKTQVTVQYMQDNGAVIPVRIHTIVISVQHNEDITLEEMRRALKEQVIRAVVPAKYLDEDTVYHLQPSGRFVIGGPQGDAGVTGRKIIVDTYGGWGAHGGGAFSGKDYTKVDRSAAYAARWVAKSLVKAGLCRRVLVQVSYAIGVAEPLSISIFTYGTSQKTERELLDVVHKNFDLRPGVIVRDLDLKKPIYQKTACYGHFGRSEFPWEVPRKLVF

  • Protein Length

    Full Length

  • Molecular Weight

    45-50 kDa

  • Endotoxin

    < 1.0 EU per μg protein as determined by the LAL method.

  • Form

    Freeze-dried powder

  • Buffer formulation

    PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.

  • Reconstitution

    Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.

  • Customization

    Site-directed mutagenesis Custom tag design Custom buffer formulation Custom full-length protein production

  • Stability Test

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.

  • Storage & Shelf Life

    Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

  • Shipping

    In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.

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Protein Description

MAT1A, or methionine adenosyltransferase 1A, is a key enzyme involved in the biosynthesis of S-adenosylmethionine (SAM), a crucial methyl donor in various biological processes, including methylation of DNA, proteins, and lipids. The significance of MAT1A in human health is underscored by its role in liver metabolism, where it regulates SAM levels and influences cellular processes such as growth and apoptosis. Dysregulation of MAT1A has been implicated in multiple liver diseases, including non-alcoholic fatty liver disease (NAFLD), hepatocellular carcinoma, and conditions associated with altered methionine metabolism. Research into recombinant MAT1A proteins has gained momentum as scientists seek to understand the enzyme's structure-function relationships, mechanisms of regulation, and how genetic variations affect its activity. These studies aim to provide insights into potential therapeutic targets for metabolic disorders and liver diseases linked to MAT1A dysfunction. By utilizing recombinant techniques to produce MAT1A, researchers can investigate its biochemical properties in detail, paving the way for novel interventions in related diseases.

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