Analytical Data
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Gene name
Tau-441/2N4R Pre-formed Fibrils
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简介
Microtubule-associated protein tau is a microtubule-associated protein found in large numbers in neurons of the central nervous system (CNS). MAPT promotes microtubule assembly and stability and may be involved in the establishment and maintenance of neuronal polarity. Overexpression of MAPT is associated with a poor prognosis of prostate cancer. MAPT has been linked to neurological disorders such as Alzheimer's and Parkinson's disease. Tau-441/2N4R Pre-formed Fibrils Protein, Human is the recombinant human-derived Tau-441/2N4R Pre-formed Fibrils protein, expressed by E. coli, with tag free.
- Application
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Alternative Names
DDPAC; FTDP-17; MAPT; MSTD; MTBT1; Tau; PHF-tau; TAU
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Species
Human
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Source
E. coli
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Tag
Tag Free
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P10636-8
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Expression Region
M1-L441
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AA Sequence
MAEPRQEFEVMEDHAGTYGLGDRKDQGGYTMHQDQEGDTDAGLKESPLQTPTEDGSEEPGSETSDAKSTPTAEDVTAPLVDEGAPGKQAAAQPHTEIPEGTTAEEAGIGDTPSLEDEAAGHVTQARMVSKSKDGTGSDDKKAKGADGKTKIATPRGAAPPGQKGQANATRIPAKTPPAPKTPPSSGEPPKSGDRSGYSSPGSPGTPGSRSRTPSLPTPPTREPKKVAVVRTPPKSPSSAKSRLQTAPVPMPDLKNVKSKIGSTENLKHQPGGGKVQIINKKLDLSNVQSKCGSKDNIKHVPGGGSVQIVYKPVDLSKVTSKCGSLGNIHHKPGGGQVEVKSEKLDFKDRVQSKIGSLDNITHVPGGGNKKIETHKLTFRENAKAKTDHGAEIVYKSPVVSGDTSPRHLSNVSSTGSIDMVDSPQLATLADEVSASLAKQGL
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Protein Length
Full Length of Isoform Tau-F
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Molecular Weight
55-65 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Tau-441/2N4R pre-formed fibrils (PFFs) represent a significant advancement in the study of tauopathies, a group of neurodegenerative disorders characterized by the accumulation of hyperphosphorylated tau protein. Tau protein plays a critical role in stabilizing microtubules in neurons; however, pathological forms of tau aggregate into fibrils, leading to synaptic dysfunction and neuronal death. The Tau-441 isoform, along with the 2N4R variant, is particularly relevant as it reflects the structural features of tau involved in neurodegenerative disease processes. Recent research has focused on the production of recombinant Tau-441/2N4R PFFs to facilitate the study of tau aggregation mechanisms and the resulting toxic effects on neuronal cells. The use of pre-formed fibrils allows researchers to investigate the propagation of tau pathology in vitro and in vivo, providing insights into how tau spreads between neurons. Understanding these mechanisms is essential for developing potential therapeutic strategies aimed at halting or reversing tau-related neurodegeneration. By harnessing the ability to create and manipulate recombinant Tau-441/2N4R PFFs, scientists are working to unravel the complex interactions that contribute to tau pathology, ultimately striving to identify effective interventions for tauopathies such as Alzheimer’s disease and other related disorders. This ongoing research emphasizes the importance of tau fibrils as a target for therapeutic discovery and underscores the significance of tau protein as a key player in neurodegenerative diseases.
