Analytical Data
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Gene name
EEF2
- Application
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Alternative Names
(EF-2)
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Species
Cricetulus griseus
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Source
E. coli
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Tag
N- His & C- Myc
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P09445
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Expression Region
1-365aa
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Molecular Weight
48.0 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Eukaryotic translation initiation factor 2 (eIF2) is a crucial protein complex involved in the initiation of protein synthesis, serving as a molecular switch that regulates translation in response to various stress signals. Recent studies have highlighted the significance of eIF2 phosphorylation in cellular stress responses, such as those induced by viral infections, oxidative stress, and nutrient deprivation. This post-translational modification controls the assembly of the eIF2-GTP-tRNA complex, thereby influencing the availability of ribosomes for translation initiation. The eIF2 pathway is intricately connected to various signaling networks, including the integrated stress response (ISR), making it a key player in cell survival and apoptosis. Due to its fundamental role in regulating protein synthesis, eIF2 is a focal point in research aimed at understanding cancer biology, neurological disorders, and metabolic diseases. Researchers are particularly interested in developing eIF2-based therapeutic strategies to modulate translation in disease contexts, leveraging recombinant eIF2 proteins for functional studies in various cellular models. Overall, the investigation of eIF2 and its recombinant proteins offers promising avenues for therapeutic interventions and deeper insights into the fundamental mechanisms of cellular homeostasis and stress responses.
