Analytical Data
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Gene name
SHP-1
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简介
SHP-1 Protein modulates signaling from tyrosine-phosphorylated cell surface receptors, including KIT and EGFR. It enhances the inhibition of mast cell activation via the Lilrb4a receptor. The SH2 regions interact with cellular components to regulate its phosphatase activity. In collaboration with MTUS1, it induces UBE2V2 expression upon angiotensin II stimulation. SHP-1 plays a crucial role in hematopoiesis. SHP-1 Protein, Human (His) is the recombinant human-derived SHP-1 protein, expressed by E. coli , with C-6*His labeled tag.
- Application
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Alternative Names
Tyrosine-Protein Phosphatase Non-Receptor Type 6; Hematopoietic Cell Protein-Tyrosine Phosphatase; Protein-Tyrosine Phosphatase 1C; PTP-1C; Protein-Tyrosine Phosphatase SHP-1; SH-PTP1; PTPN6; HCP; PTP1C
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Species
Human
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Source
E. coli
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Tag
C-6*His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
AAH02523.1
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Expression Region
K243-I541
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AA Sequence
KAGFWEEFESLQKQEVKNLHQRLEGQRPENKGKNRYKNILPFDHSRVILQGRDSNIPGSDYINANYIKNQLLGPDENAKTYIASQGCLEATVNDFWQMAWQENSRVIVMTTREVEKGRNKCVPYWPEVGMQRAYGPYSVTNCGEHDTTEYKLRTLQVSPLDNGDLIREIWHYQYLSWPDHGVPSEPGGVLSFLDQINQRQESLPHAGPIIVHCSAGIGRTGTIIVIDMLMENISTKGLDCDIDIQKTIQMVRAQRSGMVQTEAQYKFIYVAIAQFIETTKKKLEVLQSQKGQESEYGNI
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Protein Length
Partial
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Molecular Weight
33.0 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
SHP-1, or Src Homology 2 Domain-Containing Protein Tyrosine Phosphatase 1, is a crucial enzyme involved in the regulation of various cellular processes, including cell growth, differentiation, and apoptosis. It plays a significant role in immune system signaling by dephosphorylating key proteins involved in T-cell and B-cell receptor signaling pathways, thereby serving as a negative regulator of these pathways. Research on SHP-1 has increased due to its implications in autoimmune diseases, cancers, and other disorders where aberrant signaling pathways are prevalent. Moreover, SHP-1 is of particular interest because mutations or downregulation of this phosphatase have been associated with the development of hematological malignancies and resistance to therapies. The recombinant SHP-1 protein is being studied to better understand its structure-function relationships and explore its potential as a therapeutic target. Understanding the mechanisms by which SHP-1 regulates signaling pathways could provide insights into strategies for modulating immune responses and treating diseases characterized by dysregulated tyrosine phosphorylation states. Additionally, developing SHP-1 inhibitors might represent a novel approach in cancer therapy, given its role in controlling cell proliferation and survival. Thus, the ongoing research into SHP-1 not only enhances our fundamental understanding of cellular signaling and regulation but also holds promise for clinical applications in immunology and oncology.
